Distribution of anionic sites on the oviduct ciliary membrane.
Distribution of anionic sites on the oviduct ciliary membrane.
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DOI：
10.1083/jcb.72.2.482
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发表时间：
1977-02
期刊：
影响因子：
--
通讯作者：
Hein CE
中科院分区：
文献类型：
--
作者：
Anderson RG;Hein CE
用聚阳离子铁蛋白(PCF)作为输卵管睫状膜阴离子位点的直观探针。PCF与睫状膜的结合依赖于孵育液中探针的浓度。在低浓度(0.08-0.16 mg/ml)时，PCF仅与纤毛顶端结合，而在较高浓度(0.32-0.64 mg/ml)时，铁蛋白位于纤毛顶端和过渡区周围的底部，偶见散在分布于膜的其余部分。发现碱基和尖端的结合与这些区域的膜的特殊表面修饰有关。在顶端，PCF与被称为睫状冠的丝状糖萼相结合。碱基结合与一个由五到六个140-A高脊组成的系统有关，每个高脊都环绕着过渡区的膜。脊沿过渡区域的长度均匀分布(大约245A的间距)。由于用神经氨酸酶或蛋白水解酶处理输卵管阻断了探针的结合，PCF的结合部位似乎是带负电荷的糖蛋白或粘多糖。
Polycationic ferritin (PCF) was used as a visual probe for anionic sites on the oviduct ciliary membrane. The binding of PCF to ciliary membranes was dependent on the concentration of the probe in the incubation media. At low concentrations (0.08-0.16 mg/ml), PCF was bound exclusively to the tip of the cilium whereas at higher concentrations (0.32-0.64 mg/ml), ferritin was located at the tip and at the base around the transition region, with occasional scattered clumps on the remainder of the membrane. The base and tip binding was fount to be associated with special surface modifications of the membrane in these regions. At the tip, PCF was bound to a filamentous glycocalyx termed the ciliary crown. Base binding was associated with a system of five to six 140-A high ridges, each of which encircled the membrane of the transition region. The ridges were equally spaced (approxamately 245 A spacing) along the length of the transition region. Since pretreatment of oviduct with either neuraminidase or protease blocked the binding of the probe, the PCF-binding sites appear to be negatively charged glycoproteins or mucopolysaccharides.