Aging of innate immunity: functional comparisons of NK/LAK cells obtained from bulk cultures of young and aged mouse spleen cells in high concentrations of interleukin-2
Aging of innate immunity: functional comparisons of NK/LAK cells obtained from bulk cultures of young and aged mouse spleen cells in high concentrations of interleukin-2
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DOI：
10.1016/j.exger.2003.09.017
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发表时间：
2004-01-01
影响因子：
3.9
通讯作者：
Ortaldo, JR
中科院分区：
文献类型：
--
作者：
Albright, JW;Bream, JH;Ortaldo, JR
采用高浓度IL-2大量培养衰老小鼠脾细胞的技术，获得足够数量和富集的NK/LAK细胞，研究衰老对其功能的影响。其产量和富集程度与幼鼠脾细胞相当。老年小鼠和年轻小鼠NK/LAK细胞在增殖、杀伤靶细胞和产生ifn - γ的功能能力方面也相同；也就是说，它们没有表现出新鲜分离的NK/LAK细胞典型的年龄相关缺陷。然而，来自老年小鼠脾脏的NK/LAK细胞在两个方面发生了改变：(a)转录因子stat5a和5B的核易位效率发生了改变，(b)代表几种趋化因子的mRNA转录物的产生不足。我们建议谨慎使用IL-2的批量培养来获得用于衰老研究的NK/LAK细胞。然而，这项研究确实表明，衰老可能严重影响趋化因子的产生，至少在NK/LAK细胞的情况下是这样。(C) 2003 Elsevier Inc.版权所有。
The technique of bulk cultivation of aged mouse spleen cells in high concentration of IL-2 was employed to obtain NK/LAK cells in sufficient number and enrichment for studies on the effects of aging on their functions. The yield and enrichment were equivalent to that of young mouse spleen cells. The aged and young mouse NK/LAK cells were equivalent also in their functional competence to proliferate, kill target cells and produce IFN-gamma; i.e. they did not display age-associated defects typical of freshly-isolated NK/LAK cells. In two respects, however, the NK/LAK cells derived from aged mouse spleen were altered: (a) in the efficiency of nuclear translocation of transcription factors STAT 5A and 5B, and (b) in the deficiency in production of mRNA transcripts representing several chemokines. We recommend caution in the use of bulk cultivation in IL-2 to obtain NK/LAK cells for studies on aging. However, it does appear from this study that aging may severely affect chemokine production, at least in the case of NK/LAK cells. (C) 2003 Elsevier Inc. All rights reserved.