Differential fructose and glucose appetition in DBA/2, 129P3 and C57BL/6 x 129P3 hybrid mice revealed by sugar versus non-nutritive sweetener tests
Differential fructose and glucose appetition in DBA/2, 129P3 and C57BL/6 x 129P3 hybrid mice revealed by sugar versus non-nutritive sweetener tests
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DOI：
10.1016/j.physbeh.2021.113590
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发表时间：
2021-09-13
影响因子：
2.9
通讯作者：
Bodnar，Richard J.
中科院分区：
文献类型：
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作者：
Nashed，Mirna;Castillo，Alexander;Bodnar，Richard J.
近交系小鼠品系对果糖的口后食欲刺激反应（食欲）不同，如胃内（IG）糖调节和口服糖与非营养性调节实验所示。例如，FVB和SWR菌株显示出经验诱导的对8%果糖的偏好超过0.1%三氯蔗糖+0.1%糖精（S+S）溶液，而C57 BL/6（B6）和BALB/c菌株则没有。然而，所有菌株在经历之后都学会了更喜欢8%的葡萄糖而不是S +S，这归因于这种糖的强大食欲作用。本研究将该分析扩展到DBA/2（DBA）和129 P3（129）近交系小鼠。在实验1A中，随意喂养DBA和129小鼠preferredS+Sto果糖之前和之后的单独经验与两种甜味剂，表明无关的糖的产后营养作用。当食物限制（实验1B），129只小鼠继续偏爱S + S果糖，而DBA小鼠在经历后对甜味剂表现出相同的偏好，表明对果糖的食欲有一定的敏感性。在实验1C中，这两种菌株获得了显着的偏好葡萄糖超过S + S后的经验，证实了他们的敏感性，口后葡萄糖的食欲。实验2表明，C57 BL/6 × 129 P3（B6：129）杂交小鼠与近交系B6小鼠和129小鼠一样，获得了对葡萄糖的偏好，而不是对果糖的偏好。这是令人感兴趣的，因为B6：129遗传背景的甜的“味觉盲”P2 × 2 / P2 × 3双敲除（DKO）小鼠在24小时测试中更喜欢果糖而不是水，这表明果糖食欲。DKO和B6：129杂交小鼠的遗传组成差异或其他因素是否解释了DKO小鼠的果糖食欲仍有待确定。
Inbred mouse strains differ in their postoral appetite stimulating response (appetition) to fructose as demonstrated in intragastric (IG) sugar conditioning and oral sugar vs. nonnutritive conditioning experiments. For example, FVB and SWR strains show experience-induced preferences for 8% fructose over a 0.1% sucralose + 0.1% saccharin (S+S) solution, whereas C57BL/6 (B6) and BALB/c strains do not. All strains, however, learn to prefer 8% glucose toS+S after experience, which is attributed to the potent appetition actions of this sugar. The present study extended this analysis to DBA/2 (DBA) and 129P3 (129) inbred mice. In Experiment 1A, ad libitum fed DBA and 129 mice preferredS+Sto fructose before and after separate experience with the two sweeteners, indicating an indifference to the postoral nutrient effects of the sugar. When food restricted (Experiment 1B), 129 mice continued to preferS+Sto fructose while DBA mice showed equal preference for the sweeteners after experience, indicating some sensitivity to fructose appetition. In Experiment 1C, both strains acquired significant preferences for glucose overS+Safter experience, confirming their sensitivity to postoral glucose appetition. Experiment 2 revealed that C57BL/6 × 129P3 (B6:129) hybrid mice responded like inbred B6 mice and 129 mice in acquiring a preference for glucose but not fructose overS+S. This is of interest because sweet "taste-blind" P2 × 2 / P2 × 3 double-knockout (DKO) mice on a B6:129 genetic background prefer fructose to water in 24 h tests, which is indicative of fructose appetition. Whether differences in the genetic makeup of DKO and B6:129 hybrid mice or other factors explain the fructose appetition of the DKO mice remains to be determined.