A basal promoter element recognized by free RNA polymerase σ subunit determines promoter recognition by RNA polymerase holoenzyme
A basal promoter element recognized by free RNA polymerase σ subunit determines promoter recognition by RNA polymerase holoenzyme
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DOI：
10.1016/j.molcel.2006.06.010
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发表时间：
2006-07-07
期刊：
影响因子：
16
通讯作者：
Kulbachinskiy, Andrey
中科院分区：
文献类型：
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作者：
Feklistov, Andrey;Barinova, Nataliya;Kulbachinskiy, Andrey
在细菌RNA聚合酶的转录起始过程中，σ亚基识别-35和-10启动子元件;然而，游离σ不结合DNA。我们选择了强烈和特异性结合来自水生栖热菌的游离σ（A）的ssDNA适体。适体与sigma（A）的结合需要共有序列GTA（CfT）AATGGGA，其中TA（C/T）AAT片段产生的相互作用类似于RNA聚合酶全酶中-10启动子元件（共有序列TATAAT）产生的相互作用。当为dsDNA形式时，适体作为T.水生植物RNA聚合酶σ（A）全酶。基于适体的启动子的识别取决于来自适体的共同序列的下游GGGA基序，其与σ（A）区域1.2接触，并且即使在不存在-35启动子元件的情况下也指导转录起始。因此，细菌启动子的识别是由α与多个基础启动子元件的独立相互作用控制的。
During transcription initiation by bacterial RNA polymerase, the sigma subunit recognizes the -35 and -10 promoter elements; free sigma, however, does not bind DNA. We selected ssDNA aptamers that strongly and specifically bound free sigma(A) from Thermus aquaticus. A consensus sequence, GTA(CfT)AATGGGA, was required for aptamer binding to sigma(A), with the TA(C/T)AAT segment making interactions similar to those made by the -10 promoter element (consensus sequence TATAAT) in the context of RNA polymerase holoenzyme. When in dsDNA form, the aptamers function as strong promoters for the T. aquaticus RNA polymerase sigma(A) holoenzyme. Recognition of the aptamer-based promoters depends on the downstream GGGA motif from the aptamers' common sequence, which is contacted by sigma(A) region 1.2 and directs transcription initiation even in the absence of the -35 promoter element. Thus, recognition of bacterial promoters is controlled by independent interactions of a with multiple basal promoter elements.