[11C]Raclopride binding in the striatum of minimally restrained and free-walking awake mice in a positron emission tomography study
[11C]Raclopride binding in the striatum of minimally restrained and free-walking awake mice in a positron emission tomography study
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[11C]在正电子发射断层扫描研究中，雷氯必利在最小约束和自由行走的清醒小鼠纹状体中的结合
作者：
Takuwa H;Maeda J;Ikoma Y;Tokunaga M;Wakizaka H;Uchida S;Kanno I;Taniguchi J;Ito H;Higuchi M
麻醉和约束应激对脑功能有深远的影响，包括神经活动和脑血管功能，可能影响功能和神经化学正电子发射断层扫描（PET）成像数据。为了避免这种影响，我们开发了一种实验系统，通过将头部固定在支架上，在最小约束下对自由行走的清醒小鼠进行PET成像。在以下三种不同的情况下，用[11C]raclopride对d2多巴胺受体进行PET成像，研究该系统的适用性：(1)自由行走清醒状态；②1.5%异氟醚麻醉；(3)全身束缚，不麻醉。[11C]异氟醚麻醉和受限清醒状态下的raclopride binding potential （BPND）值显著低于自由行走清醒状态下（P< 0.01）。受约束清醒小鼠的心率显著高于自由行走清醒小鼠（P< 0.01），表明自由行走清醒状态使PET扫描期间的约束应激最小化。[11C]在清醒和麻醉小鼠中也进行了raclopride‐PET与甲基苯丙胺（METH）注射。甲基安非他明诱导的[11C]raclopride BPNDin麻醉小鼠的减少趋势低于自由行走清醒小鼠，这意味着自由行走清醒小鼠的PET成像可以灵敏地捕捉到多巴胺能传递的药理调节。我们的结论是，我们的系统是一个实用的活体分析平台，用于研究脑功能和神经传递元素的小动物，如那些模拟神经精神疾病。神经学报，2015。©2015 Wiley期刊公司
Anesthesia and restraint stress have profound impacts on brain functions, including neural activity and cerebrovascular function, possibly influencing functional and neurochemical positron emission tomography (PET) imaging data. For circumventing this effect, we developed an experimental system enabling PET imaging of free‐walking awake mice with minimal restraints by fixing the head to a holder. The applicability of this system was investigated by performing PET imaging of D2dopamine receptors with [11C]raclopride under the following three different conditions: (1) free‐walking awake state; (2) 1.5% isoflurane anesthesia; and (3) whole‐body restraint without anesthesia. [11C]raclopride binding potential (BPND) values under isoflurane anesthesia and restrained awake state were significantly lower than under free‐walking awake state (P< 0.01). Heart rates in restrained awake mice were significantly higher than those in free‐walking awake mice (P< 0.01), suggesting that free‐walking awake state minimized restraint stress during the PET scan. [11C] raclopride‐PET with methamphetamine (METH) injection was also performed in awake and anesthetized mice. METH‐induced reduction of [11C]raclopride BPNDin anesthetized mice showed a trend to be less than that in free‐walking awake mice, implying that pharmacological modulation of dopaminergic transmissions could be sensitively captured by PET imaging of free‐walking awake mice. We concluded that our system is of utility as anin vivoassaying platform for studies of brain functions and neurotransmission elements in small animals, such as those modeling neuropsychiatric disorders.Synapse 69:600–606, 2015. © 2015 Wiley Periodicals, Inc.