Platelet and growth factor concentrations in activated platelet-rich plasma: a comparison of seven commercial separation systems
Platelet and growth factor concentrations in activated platelet-rich plasma: a comparison of seven commercial separation systems
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DOI：
10.1007/s10047-014-0761-5
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发表时间：
2014-06-01
影响因子：
1.3
通讯作者：
Kusumoto, Kenji
中科院分区：
文献类型：
--
作者：
Kushida, Satoshi;Kakudo, Natsuko;Kusumoto, Kenji
富血小板血浆（PRP）是富含血小板的血浆。它有望在伤口愈合和再生医学（包括骨再生）等领域的临床应用。这项研究的特点是组成的PRP生产的七个商业可用的分离系统（JP 200、GLO PRP、Magellan Autoblasts Platelet Separator System、KYOCERA Medical PRP Kit、SELPHYL、MyCells和Dr. Shin's System THROMBO KIT）评价血小板、白色血细胞、红血细胞和生长因子浓度以及血小板衍生生长因子-AB（PDGF-AB），转化生长因子β 1（TGF-β 1）和血管内皮生长因子（VEGF）浓度。使用Magellan Autoblasts血小板分离系统和KYOCERA Medical PRP试剂盒制备的PRP含有最高的血小板浓度。JP 200中活化PRP的平均PDGF-AB浓度最高，其次是KYOCERA Medical PRP Kit、Magellan Autumn血小板分离系统、MyCells和GLO PRP。TGF-β 1和VEGF的浓度在个体样本中变化很大，并且与PDGF不同，不同系统之间几乎没有显著差异。SELPHYL系统产生的PRP含有低浓度的血小板和生长因子。商业PRP分离系统差异很大，熟悉其各自的优势对于将其临床应用扩展到各种条件非常重要。
Platelet-rich plasma (PRP) is blood plasma that has been enriched with platelets. It holds promise for clinical use in areas such as wound healing and regenerative medicine, including bone regeneration. This study characterized the composition of PRP produced by seven commercially available separation systems (JP200, GLO PRP, Magellan Autologous Platelet Separator System, KYOCERA Medical PRP Kit, SELPHYL, MyCells, and Dr. Shin's System THROMBO KIT) to evaluate the platelet, white blood cell, red blood cell, and growth factor concentrations, as well as platelet-derived growth factor-AB (PDGF-AB), transforming growth factor beta-1 (TGF-beta 1), and vascular endothelial growth factor (VEGF) concentrations. PRP prepared using the Magellan Autologous Platelet Separator System and the KYOCERA Medical PRP Kit contained the highest platelet concentrations. The mean PDGF-AB concentration of activated PRP was the highest from JP200, followed by the KYOCERA Medical PRP Kit, Magellan Autologous Platelet Separator System, MyCells, and GLO PRP. TGF-beta 1 and VEGF concentrations varied greatly among individual samples, and there was almost no significant difference among the different systems, unlike for PDGF. The SELPHYL system produced PRP with low concentrations of both platelets and growth factors. Commercial PRP separation systems vary widely, and familiarity with their individual advantages is important to extend their clinical application to a wide variety of conditions.