Human parainfluenza virus type 2 V protein inhibits caspase-1.
Human parainfluenza virus type 2 V protein inhibits caspase-1.
复制标题
人副流感病毒 2 型 V 蛋白抑制 caspase-1。
DOI：
10.1099/jgv.0.001037
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发表时间：
2018
影响因子：
3.8
通讯作者：
Nishio M.
中科院分区：
文献类型：
--
作者：
Ohta K;Matsumoto Y;Nishio M.
人副流感病毒2型（human parainfluenza virus type 2，hPIV 2）的多功能V蛋白在控制病毒基因组复制、抑制宿主干扰素应答和促进病毒生长等方面发挥重要作用。我们筛选了酵母双杂交文库，使用V蛋白作为诱饵，以确定宿主因子，是重要的其他功能的V.从HeLa细胞来源的cDNA文库中分离的几个阳性克隆之一编码caspase-1。我们发现，在哺乳动物细胞中，V的C-末端区域与caspase-1的C-末端区域相互作用。此外，V蛋白以剂量依赖性方式抑制caspase-1活性和白细胞介素-1 β（IL-1β）的形成。在人单核细胞THP-1细胞中由野生型hPIV 2感染诱导的IL-1β分泌显著低于由缺乏V蛋白或具有突变V的重组hPIV 2诱导的IL-1 β分泌。这些数据表明，hPIV 2 V蛋白通过其与半胱天冬酶-1的相互作用抑制半胱天冬酶-1介导的IL-1β成熟。
The multifunctional V protein of human parainfluenza virus type 2 (hPIV2) plays important roles in controlling viral genome replication, inhibiting the host interferon response and promoting virus growth. We screened a yeast two-hybrid library using V protein as bait to identify host factors that are important for other functions of V. One of several positive clones isolated from HeLa cell-derived cDNA library encodes caspase-1. We found that the C-terminal region of V interacts with the C-terminal region of caspase-1 in mammalian cells. Moreover, the V protein repressed caspase-1 activity and the formation of interleukin-1β (IL-1β) in a dose-dependent manner. IL-1β secretion induced by wild-type hPIV2 infection in human monocytic THP-1 cells was significantly lower than that induced by recombinant hPIV2 lacking V protein or having a mutant V. These data suggest that hPIV2 V protein inhibits caspase-1-mediated maturation of IL-1β via its interaction with caspase-1.