REPETITIVE DICTYOSTELIUM HEAT-SHOCK PROMOTOR FUNCTIONS IN SACCHAROMYCES-CEREVISIAE
REPETITIVE DICTYOSTELIUM HEAT-SHOCK PROMOTOR FUNCTIONS IN SACCHAROMYCES-CEREVISIAE
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DOI：
10.1128/mcb.4.4.591
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发表时间：
1984-01-01
影响因子：
5.3
通讯作者：
LODISH, HF
中科院分区：
文献类型：
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作者：
CAPPELLO, J;ZUKER, C;LODISH, HF
网骨藻基因组包含40个拷贝的4.7 kb重复和明显可转座的DNA序列（DIRS-1）和大约250个较小的元件，这些元件似乎是DIRS-1的缺失或重排。这些序列的转录物在分化过程中被诱导，并且也可以被生长细胞的热休克处理。一个这样的克隆元件pB41.6（2.5kb）含有与果蝇共有热休克启动子相同的核苷酸序列。为了测试该序列是否真的可以控制DIRS-1相关RNA的表达，将该基因组片段克隆到酵母细胞中。在酵母细胞中，41.6指导1.7 kb RNA的合成，热休克诱导至少10倍。转录起始于推定的启动子序列的约124个碱基3“处，并终止于41.6个插入物内。一个381个碱基对的亚克隆，包含推定的启动子序列是足以诱导热休克反应的酵母细胞中的41.6。
The Dictyostelium genome contains 40 copies of a 4.7-kilobase [kb] repetitive and apparently transposable DNA sequence (DIRS-1) and about 250 smaller elements that appear to be deletions or rearrangements of DIRS-1. Transcripts of these sequences are induced during differentiation and also be heat shock treatment of growing cells. One such cloned element, pB41.6 (2.5 kb) contains a nucleotide sequence identical to the Drosophila consensus heat shock promotor. To test whether this sequence might indeed control the expression of DIRS-1-related RNA, this genomic segment was cloned into yeast cells. In yeast cells, 41.6 directs synthesis of a 1.7-kb RNA that is induced at least 10-fold by heat shock. Transcription initiates at about 124 bases 3'' of the putative promotor sequence and terminates within the 41.6 insert. A 381-base-pair subclone that contains the putative promotor sequence is sufficient to induce the heat shock response of 41.6 in yeast cells.