The aging of hematopoietic stem cells
The aging of hematopoietic stem cells
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DOI：
10.1038/nm0996-1011
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发表时间：
1996-09-01
期刊：
影响因子：
82.9
通讯作者：
Weissman, IL
中科院分区：
文献类型：
--
作者：
Morrison, SJ;Wandycz, AM;Weissman, IL
我们从老年小鼠的骨髓中纯化了造血干细胞（HSCs），并将其特性与年轻和中年小鼠的HSCs进行了比较。单一的，重建HSC（有限稀释）从老年和年轻的小鼠表现出不可区分的祖细胞在体内的活动。HSC在老年小鼠骨髓中的频率是老年小鼠的五倍;然而，来自老年小鼠的HSC在归巢和移植受辐射者的骨髓中的效率只有四分之一。青年和中年小鼠的HSC很少处于细胞周期的S/C-2/M期，而老年小鼠的HSC经常处于周期中。我们推测老年小鼠造血干细胞的意外增殖可能与老年小鼠白血病发病率的增加有关。HSC随着年龄的增长而变化，但目前尚不清楚这些变化是内在决定的还是由环境老化引起的。
We have purified hematopoietic stem cells (HSCs) from the bone marrow of old mice and compared their properties to HSCs in young and middle-aged mice. Single, reconstituting HSCs (by limit dilution) from old and young mice exhibited indistinguishable progenitor activities in vivo. HSCs were five times as frequent in the bone marrow of old mice; however, HSCs from old mice were only one-quarter as efficient at homing to and engrafting the bone marrow of irradiated recipients. HSCs in young and middle-aged mice rarely were in the S/C-2/M phases of the cell cycle, but HSCs in old mice were frequently in cycle. We speculate that the unexpected proliferation of HSCs in old mice might be related to the increased incidence of leukemia in old mice. HSCs change with age, but it is unknown whether these changes are determined intrinsically or caused by the aging of their environment.