THE REGULATION OF CIRCADIAN PERIOD BY PHOTOTRANSDUCTION PATHWAYS IN ARABIDOPSIS
THE REGULATION OF CIRCADIAN PERIOD BY PHOTOTRANSDUCTION PATHWAYS IN ARABIDOPSIS
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DOI：
10.1126/science.7855596
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发表时间：
1995-02-24
期刊：
影响因子：
56.9
通讯作者：
KAY, SA
中科院分区：
文献类型：
--
作者：
MILLAR, AJ;STRAUME, M;KAY, SA
表达荧光素酶基因与昼夜节律调控启动子融合的转基因拟南芥植株表现出强烈的生物发光节律。这种循环发光在白光下有24.7小时的周期，但在恒定的黑暗中有30到36小时的周期。无论是红光还是蓝光，野生型的周期都缩短到了25小时。缺乏光敏色素的突变延长了在连续红光下的周期，但在连续的蓝色战斗中几乎没有影响，而携带突变的幼苗在黑暗中激活依赖光的通路，在持续的黑暗中保持较短的周期。这些结果表明，光敏色素和蓝色光感受器通路都控制着生物钟的周期。
Transgenic Arabidopsis plants expressing a luciferase gene fused to a circadian-regulated promoter exhibited robust rhythms in bioluminescence. The cyclic luminescence has a 24.7-hour period in white light but 30- to 36-hour periods under constant darkness. Either red or blue light shortened the period of the wild type to 25 hours. A phytochrome-deficient mutation lengthened the period in continuous red light but had little effect in continuous blue fight, whereas seedlings carrying mutations that activate light-dependent pathways in darkness maintained shorter periods in constant darkness. These results suggest that both phytochrome- and blue light-responsive photoreceptor pathways control the period of the circadian clock.