MiR-4728-3p could act as a marker of HER2 status
MiR-4728-3p could act as a marker of HER2 status
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MiR-4728-3p 可以作为 HER2 状态的标记。
DOI：
10.3233/cbm-150524
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发表时间：
2015-01-01
影响因子：
3.1
通讯作者：
Liu, Ping
中科院分区：
文献类型：
--
作者：
Li, Hai;Zhou, Xin;Liu, Ping
背景技术背景：miR-4728最近在几种细胞系和有限的组织样本中被鉴定为与HER 2相关。目的：研究miR-4728是否可以在更大的队列中预测HER 2状态。miR-4728- 3 p和miR-4728- 5 p在乳腺癌（BC）和胃癌（GC）中表达使用定量逆转录聚合酶链反应（qRT-PCR）和荧光原位杂交（FISH）检测不同HER 2状态的组织。结果：miR-4728- 3 p和miR-4728- 5 p在HER 2阳性患者中的表达显著高于HER 2阴性患者。与癌旁正常组织相比，miR-4728- 3 p和miR-4728- 5 p在HER 2阴性和HER 2阳性胃癌组织中均升高，而在HER 2阳性癌组织中仅miR-4728- 3 p升高。进一步分析显示，miR-4728- 3 p在区分BC和GC患者中具有不同HER 2染色强度的亚组方面具有比miR-4728- 5 p更大的能力。结论：miR-4728 - 3 p比miR-4728 - 5 p更能区分不同HER 2状态的乳腺癌患者。血浆miR-4728- 3 p可能作为一种非侵入性的生物标志物预测HER 2状态。
BACKGROUND: MiR-4728 was recently identified to be related with HER2 in several cell lines and limited tissue samples.OBJECTIVE: To investigate whether miR-4728 could predict HER2 status in a larger cohort.METHODS: The expression of miR-4728-3p and miR-4728-5p was identified in breast cancer (BC) and gastric cancer (GC) tissues with different HER2 status using quantitative reverse transcription polymerase chain reaction (qRT-PCR) and fluorescence in situ hybridization (FISH). An additional 22 plasma samples was investigated to explore the potential application of miR-4728 as a non-invasive biomarker in predicting HER2 status.RESULTS: MiR-4728-3p and miR-4728-5p were significantly up-regulated in HER2-positive patients compared with HER2-negative patients. Compared with the expression in adjacent normal tissues, miR-4728-3p and miR-4728-5p were both elevated in HER2-negative and HER2-positive GC tissues but only miR-4728-3p in HER2-positive BC tissues. Further analyses revealed that miR-4728-3p had greater ability than miR-4728-5p in discriminating subgroups with different intensity of HER2 staining in both BC and GC patients. In addition, miR-4728-3p but not miR-4728-5p was significantly up-regulated in plasma of BC patients with positive HER2.CONCLUSIONS: MiR-4728-3p had better ability in distinguishing patients with different status of HER2 than miR-4728-5p. And plasma miR-4728-3p might act as a non-invasive biomarker in predicting HER2 status.