GENERATION AND ANALYSIS OF NONHOMOLOGOUS RNA-RNA RECOMBINANTS IN BROME MOSAIC-VIRUS - SEQUENCE COMPLEMENTARITIES AT CROSSOVER SITES
GENERATION AND ANALYSIS OF NONHOMOLOGOUS RNA-RNA RECOMBINANTS IN BROME MOSAIC-VIRUS - SEQUENCE COMPLEMENTARITIES AT CROSSOVER SITES
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DOI：
10.1128/jvi.65.8.4153-4159.1991
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发表时间：
1991-08-01
影响因子：
5.4
通讯作者：
DZIANOTT, AM
中科院分区：
文献类型：
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作者：
BUJARSKI, JJ;DZIANOTT, AM
雀麦花叶病毒（BMV）基因组的所有三个单链RNA都含有高度保守的193个碱基的3'非编码区。 为了研究单个BMV RNA组分之间的重组，用体外转录的野生型BMV RNA 1和2以及在3'端附近携带缺失的RNA 3突变体的混合物感染大麦植物。 这产生了同源和非同源3'重组BMV RNA3变体的群体。 测序结果表明，这些重组体是通过与BMV RNA1或RNA2的单或双交换而获得的。 重组连接处的一级序列未显示出任何相似性。 然而，它们可以对齐以形成双链异源双链体。 这表明BMV RNA之间的局部杂交可能支持分子间交换。
All three single-stranded RNAs of the brome mosaic virus (BMV) genome contain a highly conserved, 193-base 3' noncoding region. To study the recombination between individual BMV RNA components, barley plants were infected with a mixture of in vitro-transcribed wild-type BMV RNAs 1 and 2 and an RNA3 mutant that carried a deletion near the 3' end. This generated a population of both homologous and nonhomologous 3' recombinant BMV RNA3 variants. Sequencing revealed that these recombinants were derived by either single or double crossovers with BMV RNA1 or RNA2. The primary sequences at recombinant junctions did not show any similarity. However, they could be aligned to form double-stranded heteroduplexes. This suggested that local hybridizations among BMV RNAs may support intermolecular exchanges.