ACCUMULATION OF ABSCISIC-ACID IN CELL-SUSPENSION CULTURES OF OILSEED RAPE TREATED WITH THE GROWTH RETARDANT BAS 111..W - EFFECTS ON OSMOTIC POTENTIAL AND POTASSIUM, WATER AND SUGAR CONTENTS
ACCUMULATION OF ABSCISIC-ACID IN CELL-SUSPENSION CULTURES OF OILSEED RAPE TREATED WITH THE GROWTH RETARDANT BAS 111..W - EFFECTS ON OSMOTIC POTENTIAL AND POTASSIUM, WATER AND SUGAR CONTENTS
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DOI：
10.1016/s0176-1617(11)81033-3
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发表时间：
1992-11-01
影响因子：
4.3
通讯作者：
GROSSMANN, K
中科院分区：
文献类型：
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作者：
HAUSER, C;KWIATKOWSKI, J;GROSSMANN, K
油菜细胞悬浮培养的研究CV. Kasan）在指数生长期开始时细胞脱落酸（阿坝）含量短暂增加，继代培养5天后达到最大值。与内源水平成比例，A-BA分泌到培养基中。用10（-4）mol . L-1氟啶酮，阿坝生物合成的抑制剂，抑制A-BA水平的上升几乎完全。因此，阿坝可能是响应于在具有较高糖和矿物质浓度的新鲜培养基中传代培养细胞所产生的渗透胁迫而产生的。这一假设得到了以下发现的支持，即内源阿坝积累与培养基中甘露醇诱导的渗透胁迫增加（从-0.39到-0.74 MPa）相关。同时，细胞含水量和细胞液的渗透势下降。三唑类生长延缓剂BAS 111的应用。W还增加了内源阿坝的量，但没有改变介质的渗透势。该化合物被认为能抑制阿坝催化剂转化为菜豆酸。与此相反，环己烷三酮型阻滞剂调环二酮钙和LAB 198999没有改变A-BA含量显着。BAS 111处理后5天获得的剂量反应曲线。结果表明，A-BA在细胞中的积累与细胞液中钾、水分含量和渗透势的升高有关。后一种效应可以通过BAS 111中蔗糖和/或其水解产物葡萄糖和果糖的限制性摄取来解释。W处理的悬浮细胞。以细胞数和细胞干重计算，经10（-5）~ 10（-4）mol . L-1 BAS 111. W.还发现镁显著增加，而钙仅略微升高，钠水平保持不变。BAS 111介导的内源阿坝水平升高引起的钾和水分含量的变化。因此，W相反的阿坝增加时，观察到的渗透胁迫。阿坝对这些参数的具体影响，因此可以阐明不干扰的同时水分胁迫的影响。据推测，延迟引起的阿坝积累影响内源钾，水和蔗糖水平和细胞汁液的渗透势。
Cell suspension cultures of oilseed rape (Brassica napus L. cv. Kasan) showed a transient increase in cellular abscisic acid (ABA) content at the beginning of the exponential growth phase, with a maximum after 5 days of subculture. In proportion to the endogenous levels, A-BA was excreted into the culture medium. Treatment with 10(-4) mol . L-1 fluridone, an inhibitor of ABA biosynthesis, suppressed the rise in A-BA levels almost completely. Thus, ABA might be produced in response to osmotic stress resulting from subculturing the cells in fresh medium with higher sugar and mineral concentrations. This hypothesis was supported by the finding that endogenous ABA accumulation correlated with increasing osmotic stress induced by mannitol (from - .39 to -0.74 MPa) in the medium. Concomitantly, cellular water content and the osmotic potential of the cell sap decreased. Application of the triazole-type growth retardant BAS 111. W also increased the amount of endogenous ABA, but without changes in the osmotic potential of the medium. The compound is thought to inhibit ABA catabolism to phaseic acid. In contrast, the cyclohexanetrione-type retardants prohexadione calcium and LAB 198999 did not alter A-BA contents significantly. Dose response curves obtained 5 days after treatment with BAS 111. W revealed that A-BA accumulation in the cells was accompanied by rising potassium and water levels and osmotic potential of the cell sap. The latter effect could be explained by a restricted uptake of sucrose and/or its hydrolysis products glucose and fructose, in BAS 111..W treated suspension cells. Calculated on a cell number and cell dry weight basis, ABA, potassium and water content increased to approximately 210, 160 and 140 %, respectively, of the control values after treatment with 10(-5) to 10(-4)mol . L-1 BAS 111..W. A significant increase was also found in magnesium while calcium was only slightly elevated and sodium levels remained unchanged. The changes in the potassium and water contents following the elevated endogenous ABA levels mediated by BAS 111..W were thus opposite to those observed when ABA was increased in response to osmotic stress. The specific effects of ABA on these parameters may thus be elucidated without interference from the effects of simultaneous water stress. It is speculated that retardant-caused ABA accumulation influences endogenous potassium, water and sucrose levels and the osmotic potential of the cell sap.