Spermatogenesis and steroidogenesis in mouse, hamster and monkey testicular tissue after cryopreservation and heterotopic grafting to castrated hosts
Spermatogenesis and steroidogenesis in mouse, hamster and monkey testicular tissue after cryopreservation and heterotopic grafting to castrated hosts
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DOI：
10.1530/rep.0.1240339
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发表时间：
2002-09-01
期刊：
影响因子：
3.8
通讯作者：
Gosden, R
中科院分区：
文献类型：
--
作者：
Schlatt, S;Kim, SS;Gosden, R
睾丸组织的检索、体外储存和自体移植可能成为保护男性性腺功能的重要策略。本研究使用同基因和免疫缺陷裸鼠作为宿主，以及未成熟和成年小鼠、新生和成年光退行的准加仓鼠和新生狨猴，以确定睾丸组织移植维持睾丸形态和功能完整性的潜力。睾丸组织被皮下移植。无论是作为新鲜组织还是在冷冻保存到成年、兰花切除宿主体内后。几天后，接受啮齿动物睾丸组织的小鼠进行尸检，并收集血液样本。百分之六十五的小鼠同种移植物含有形态正常的睾丸组织和生精小管，并具有一定程度的生精恢复。酶解后回收成熟精子。尽管成年小鼠和仓鼠组织中精子发生的恢复有限，但在未成熟啮齿动物的移植物中观察到完整的精子发生。异种移植后第 135 天，新生狨猴的睾丸组织发育至精母细胞阶段。在完整对照小鼠和接受新鲜小鼠和仓鼠移植物的小鼠中，雄激素浓度相当，在接受冷冻保存移植物和成年光退化仓鼠组织的小鼠中略低，在去势对照小鼠和接受狨猴组织的小鼠中较低。这些结果表明，未成熟和成年睾丸组织的同种移植物和异种移植物作为皮下移植物变得功能活跃。这种方法可能与冷冻保存相结合，作为储存和激活雄性生殖系以及患者雄激素替代疗法的工具。
Retrieval, extracorporal storage and autotransplantation of testicular tissue could become an important strategy for preserving male gonadal function. The present study used syngeneic and immunodeficient nude mice as hosts, and immature and adult mice, neonatal, and adult photoregressed Djungarian hamsters and neonatal marmosets to identify the potential of testicular tissue grafting to maintain the morphological and functional integrity of the testis. Testicular tissue was grafted s.c. either as fresh tissue or after cryopreservation into adult, orchidectornized hosts. The mice that received rodent testis tissue were autopsied So days later, and blood samples were collected. Sixty-five per cent of mouse isografts contained morphologically normal testicular tissue and seminiferous tubules with some degree of spermatogenic recovery. Mature spermatozoa were recovered after enzymatic disaggregation. Although the recovery of spermatogenesis was limited in adult mouse and hamster tissue, complete spermatogenesis was observed in grafts from immature rodents. Testicular tissue from neonatal marmosets developed up to the stage of spermatocytes at day 135 after xenografting. Androgen concentrations were comparable in intact control mice and in mice receiving fresh mouse and hamster grafts, slightly lower in mice receiving cryopreserved grafts and adult photoregressed hamster tissue, and low in castrated control mice and in mice receiving marmoset tissue. These results show that isografts and xenografts of immature and adult testicular tissue become functionally active as a s.c. graft in the mouse and that this approach might he useful in combination with cryopreservation as a tool for storage and activation of the male germ line and androgen replacement therapy in patients.