In vitro biofilm forming potential of Streptococcus suis isolated from human and swine in China
In vitro biofilm forming potential of Streptococcus suis isolated from human and swine in China
复制标题
中国从人和猪中分离的猪链球菌的体外生物膜形成潜力
DOI：
10.1590/s1517-83822012000300021
复制
发表时间：
2012-09-01
影响因子：
2.2
通讯作者：
Chengping, Lu
中科院分区：
文献类型：
--
作者：
Dawei, Guo;Liping, Wang;Chengping, Lu
猪链球菌是猪的病原体，也是一种人畜共患病。生物膜的形成使S.猪成为持久的殖民者并抵抗宿主免疫系统和抗生素的清除。研究了不同菌株的挂膜能力。用激光共聚焦显微镜（CLSM）、扫描电镜（SEM）和组织培养板结晶紫染色等方法对分离株进行了鉴定。此外，在本研究中，五种抗菌剂对生物膜形成的影响进行了测定。S.猪在光滑和粗糙表面上均产生生物膜。生长培养基中的营养成分包括葡萄糖和NaCl调节生物膜的形成。46株S的生物膜形成能力存在显著差异。猪种。S.的生物膜形成潜力。血清9型较血清2型及其它各型强。青霉素、红霉素、阿奇霉素、环丙沙星和氧氟沙星5种常用抗菌药物在亚抑菌浓度下均能抑制生物膜形成，其中环丙沙星和氧氟沙星的抑制作用较强。我们的研究提供了一个详细的分析，生物膜形成的潜力，在S。猪链球菌的研究，是了解其致病作用的一个步骤，并最终导致更好地了解如何消除S。猪在抗生素治疗下以生物膜形式生长。
Streptococcus suis is a swine pathogen and also a zoonotic agent. The formation of biofilms allows S. suis to become persistent colonizers and resist clearance by the host immune system and antibiotics. In this study, biofilm forming potentials of various S. suis strains were characterized by confocal laser scanning microscopy (CLSM), scanning electron microscopy (SEM) and tissue culture plates stained with crystal violet. In addition, the effects of five antimicrobial agents on biofilm formation were assayed in this study. S. suis produced biofilms on smooth and rough surface. The nutritional contents including glucose and NaCl in the growth medium modulated biofilm formation. There was a significant difference in their biofilm-forming ability among all 46 S. suis strains. The biofilm-forming potential of S. suis serotype 9 was stronger than type 2 and all other types. However, biofilm formation was inhibited by five commonly used antimicrobial agents, penicillin, erythromycin, azithromycin, ciprofloxacin, and ofloxacin at subinhibitory concentrations, among which inhibition of ciprofloxacin and ofloxacin was stronger than that of other three antimicrobial agents. Our study provides a detailed analysis of biofilm formation potential in S. suis, which is a step towards understanding its role in pathogenesis, and eventually lead to a better understanding of how to eradicate S. suis growing as biofilms with antibiotic therapy.