Calcium and ethylene glycol tetraacetic acid mitigate toxicity and alteration of gene expression associated with cadmium stress in chickpea (Cicer arietinum L.) shoots
Calcium and ethylene glycol tetraacetic acid mitigate toxicity and alteration of gene expression associated with cadmium stress in chickpea (Cicer arietinum L.) shoots
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DOI：
10.1007/s00709-020-01605-x
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发表时间：
2021-01
期刊：
影响因子：
2.9
通讯作者：
Lamia Sakouhi;O. Kharbech;Marouane Ben Massoud;C. Gharsallah;S. B. Hassine;S. Munemasa;Y. Murata;A. Chaoui
中科院分区：
文献类型：
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作者：
Lamia Sakouhi;O. Kharbech;Marouane Ben Massoud;C. Gharsallah;S. B. Hassine;S. Munemasa;Y. Murata;A. Chaoui
为了探讨钙（Ca）和乙二醇四乙酸（EGTA）对镉（Cd）胁迫下鹰嘴豆（Cicer arietinumL.）将种子暴露于200 μM Cd胁迫6天或3天，然后将金属与100 mM CaCl 2或100 μM EGTA共处理3天。Ca和EGTA的添加促进了幼苗的生长。这种保护作用与金属诱导的氧化应激的缓解相关，例如过氧化氢（H2 O2）含量的减少。此外，钙和EGTA刺激硫氧还蛋白（Trx）和硫氧还蛋白还原酶（NTR）的活动（2.75-和1.75-倍增加时相比，镉胁迫，分别）保护，从而保护蛋白质-SH基团从镉介导的氧化，并调制铁氧还蛋白（Fdx）的活性到对照水平。此外，钙和EGTA恢复谷胱甘肽氧化还原稳态，主要是通过保持高水平的谷胱甘肽还原型（GSH）。这一效应与Cd刺激的谷胱甘肽还原酶（GR）活性的维持和谷胱甘肽过氧化物酶（GPX，比Cd胁迫芽低43%）活性的下降相一致。Ca和EGTA可抵消Cd对Cu/Zn-SOD同工酶活性和基因表达的抑制作用，并调节过氧化氢酶（CAT）和抗坏血酸过氧化物酶（APX）活性。总体而言，我们的研究结果提供了证据，钙和EGTA补充可能是一个有前途的方法在镉污染的环境修复。
In the aim to estimate the protective role of calcium (Ca) and ethylene glycol tetraacetic acid (EGTA) against cadmium (Cd)–induced damage, chickpea (Cicer arietinumL.) seeds were exposed to 200 μM Cd stress for 6 days or 3 days then subjected to co-treatment of the metal with either 100 mM CaCl2or 100 μM EGTA for 3 additional days. The addition of Ca and EGTA improved seedling growth. This protecting effect was correlated to the alleviation of the metal-induced oxidative stress, exemplified by the reduction of hydrogen peroxide (H2O2) contents. Besides, Ca and EGTA stimulated thioredoxin (Trx) and thioredoxin reductase (NTR) activities (2.75- and 1.75-fold increase when compared to Cd-stressed, respectively) protecting, thereby, protein –SH groups from the Cd-mediated oxidation, and modulated ferredoxin (Fdx) activity to a control level. Moreover, Ca and EGTA reinstated the glutathione redox steady state, mainly via preserving a high level of glutathione reduced form (GSH). This effect coincided with the maintaining of the Cd-stimulated glutathione reductase (GR) activity and the decline of glutathione peroxidase (GPX, 43% lower than Cd-stressed shoots) activity. Ca and EGTA counteracted the inhibitory effect of Cd on the activity and gene expression of Cu/Zn-superoxide dismutase (Cu/Zn-SOD) isoenzyme and modulated the activities of catalase (CAT) and ascorbate peroxidase (APX). Overall, our results provided evidence that Ca and EGTA supplement could be a promising approach in the remediation of Cd-contaminated environment.