Lipopolysaccharide promotes and augments metal allergies in mice, dependent on innate immunity and histidine decarboxylase
Lipopolysaccharide promotes and augments metal allergies in mice, dependent on innate immunity and histidine decarboxylase
复制标题
DOI：
10.1111/j.1365-2222.2007.02705.x
复制
发表时间：
2007-05-01
影响因子：
6.1
通讯作者：
Endo, Y.
中科院分区：
文献类型：
--
作者：
Sato, N.;Kinbara, M.;Endo, Y.
背景金属过敏的小鼠模型很少，镍过敏的小鼠尤其难以诱导。目的研究脂多糖（LPS）对小鼠镍及其他金属过敏的影响。方法用金属盐和LPS致敏后10天，用相同的金属盐激发小鼠耳。结果LPS+ NiCl 2（1 mM）可有效地使小鼠对镍过敏，LPS在非常低的浓度下有效，无论是皮内注射还是腹膜内注射。C57 BL/6小鼠对Ni的耳肿胀反应比BALB/c小鼠更严重和更快。在肥大细胞缺陷型小鼠、TNF-α缺陷型小鼠以及有趣的是，甚至在裸（T细胞缺陷型）小鼠中，NiCl 2 +LPS诱导的Ni过敏的程度与相应对照小鼠中的Ni过敏的程度相似，但是在TLR 4突变型小鼠、巨噬细胞耗尽型小鼠和IL-1缺陷型小鼠中，它仅微弱地诱导Ni过敏。耳中组胺形成酶组氨酸脱羧酶（HDC）的活性与耳肿胀平行增加，且HDC缺陷小鼠对耳肿胀具有抵抗力。用NiCl 2 +LPS激发增强耳肿胀（相对于单独的NiCl 2）。结论LPS是小鼠金属过敏的重要诱导剂，并能有效地促进金属过敏（依赖于天然免疫和肥大细胞以外的细胞中HDC的诱导）。我们讨论了细菌环境对金属过敏的建立及其激发的重要性，以及目前的想法（包括T细胞的贡献）应该在未来的研究中重新评估。
Background Few adequate murine models exist for metal allergies, it being especially difficult to induce Ni allergy in mice.Objective We examined the effect of lipopolysaccharide (LPS) on allergies to Ni and other metals in mice.Methods Ten days after sensitization with a metal salt and LPS, the ears were challenged with the same metal salt.Results LPS+NiCl2 (1 mM) was effective at sensitizing mice to Ni, LPS being effective at very low concentrations whether injected intradermally or intraperitoneally. The ear-swelling response to Ni was more severe and more rapid in C57BL/6 mice than in BALB/c mice. In mast-cell-deficient mice, TNF-alpha-deficient mice, and interestingly even in nude (T cell deficient) mice, NiCl2+LPS induced a Ni allergy similar in degree to that in the respective control mice, but it induced Ni allergy only weakly in TLR4-mutant mice, macrophage-depleted mice, and IL-1-deficient mice. The activity of the histamine-forming enzyme histidine decarboxylase (HDC) in the ears increased in parallel with ear swelling, and HDC-deficient mice were resistant to ear swelling. Challenge with NiCl2+LPS augmented ear swelling (vs. NiCl2 alone). LPS induced effective sensitization to other metals (Cr, Co, Pd, or Ag).Conclusions These results indicate that in mice, LPS is a very important inducer of metal allergies, and potently promotes them (dependent on both innate immunity and HDC induction in cells other than mast cells). We discussed the idea that the bacterial environment is important for the establishment of metal allergies and for their provocation, and that the current thinking (including the contribution of T cells) should be reappraised in future studies.