A Method for Collecting the Blood from the Suprarenfal Gland in the Dog, without Fastening, Narcotizing, Laparotomy or Provoking any Pain
A Method for Collecting the Blood from the Suprarenfal Gland in the Dog, without Fastening, Narcotizing, Laparotomy or Provoking any Pain
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一种不紧固、不麻醉、不剖腹、不引起任何疼痛的狗肾上腺采血方法
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通讯作者：
M. Watanabé
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作者：
Y. Satake;T. Sugawara;M. Watanabé
本文描述了一种从随意站立、坐着或睡觉的狗的肾上腺上腺采集血液的方法。即通过犬腰路手术采血，手术野事先受相应背根干扰脱敏，真正做到不系、不麻醉、不开腹、不引起任何疼痛。2。用本方法和兔肠段法估计的犬肾上腺素的自发释放量，即除练习本方法外不进行任何操作且动物不兴奋时的排出量，确定一个腺体为0.00001-0.00012毫克。，平均0.00003（左）0.00004（右）毫克。每公斤、每分钟和0.00007毫克。对于两个腺体，被测定的血流量平均为每公斤每分钟0.3毫升，肾上腺素浓度平均为0.0001毫克。每1毫升肾上静脉血液。因此，用这种新方法测定的肾上腺素输出量，与用cava法测定的相同性质的数字相比，确实有很大的不同。口袋法在非麻醉、去传入的狗和麻醉、正常的狗，在本实验室以前的论文中提出。这种方法所获得的肾上腺素释放量小得惊人，这是因为它所获得的肾上腺素在肾上腺上静脉血液中的浓度很低。3。通过对未麻醉、去传入、但系住的犬进行的实验，也明确地证明了腔袋的准备会引起肾上腺素输出的显著增加，并将同一只犬腰路手术的结果进行了比较。通过使用麻醉，这种差异大大减少，最后，当建立足够深的麻醉时，这种差异完全消失。
I. A method for collecting the blood from the suprarenal gland of the dog that is standing, sitting or sleeping at will is described in the text. Namely the blood is collected through the lumbar route operation in the dog, the operation field of which is beforehand desensitized by the interference of the corresponding dorsal roots, and really without fastening, narcotizing, laparotomy, or evoking any pain. II. The spontaneous liberation of epinephrine in dogs estimated by the use of this method and the rabbit intestine segment method, that is the discharge when no manipulation other than practising this method itself is conducted and the animal is not excited, is determined for one gland as 0.00001-0.00012 mgrm., on an average 0.00003 (for the left) 0.00004 (for the right) mgrm. per kilo and per minute and 0.00007 mgrm. for the both glands together, the blood flow being assayed mean 0.3 c. c. for one gland per kilo and per minute, and the epinephrine concentration as mean 0.0001 mgrm. per 1 c. c. suprarenal vein blood. The epinephrine output determined by this new method is therefore really of a quite different order compared with the figures of the same nature obtained by the cava. pocket method in the non-anaesthetized, de-afferented dogs as well as in the anaesthetized, normal dogs, presented in the previous papers from this laboratory. The striking smallness of the epinephrine liberation taken by this method is in virtue of the low concentration of epinephrine in the suprarenal vein blood obtained by it. III. By some experiments with the non-anaesthetized, de-afferented, but fastend dogs, it was also definitely demonstrated that the preparing of the cava pocket induces a remarkable exaggeration of the epinephrine output, the results with the lumbar route operation in one and the same dog being brought forward for comparison. By the use of anaesthesia this difference becomes much reduced, and finally it disappears completely when sufficiently deep anaesthesia is established.