FREE-RADICAL INVOLVEMENT IN THE OXIDATIVE PHENOMENA INDUCED BY TERT-BUTYL HYDROPEROXIDE IN ERYTHROCYTES
FREE-RADICAL INVOLVEMENT IN THE OXIDATIVE PHENOMENA INDUCED BY TERT-BUTYL HYDROPEROXIDE IN ERYTHROCYTES
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DOI：
10.1016/0304-4165(83)90183-6
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发表时间：
1983-01-01
期刊：
影响因子：
--
通讯作者：
STERN, A
中科院分区：
文献类型：
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作者：
THORNALLEY, PJ;TROTTA, RJ;STERN, A
通过使用自旋捕获 5,5-二甲基-1-吡咯啉-N-氧化物 (DMPO) 的 ESR 技术，证明自由基参与[人]红细胞中叔丁基过氧化氢诱导的氧化事件。研究了叔丁基过氧化氢与血红蛋白和完整细胞系统的反应。含有HbO2的系统显示出仅产生DMPO的叔丁氧基自由基自旋加合物(DMPO-OBut)，这表明叔丁氧基自由基的产生。 含高铁血红蛋白的系统显示出 DMPO 氧化衍生物 5,5-二甲基-2-酮吡咯烷-1-氧基 (DMPOX) 的产生，该衍生物先前与高度氧化的血红素-Fe 的生成有关。含有 HbCO 的系统显示出 DMPO-OBut 和 DMPOX 的产生，但明显慢于其他任何一个 Hb 系统。一般来说，血红蛋白系统中自由基的产生比完整细胞系统中的更快，这表明叔丁基氢过氧化物介导的作用存在膜转运限速步骤。使用自由基清除剂抑制 DMPO-OBut 产生的数据与清除剂对叔丁氧基自由基的已知反应性一致。叔丁氧基自由基和血红素中间体的重要作用分别与叔丁基过氧化氢诱导的红细胞脂质过氧化和血红蛋白氧化有关。
Free radical involvement in the oxidative events induced by tert-butyl hydroperoxide in [human] erythrocytes was demonstrated by the use of the ESR technique of spin trapping with the spin trap 5,5-dimethyl-1-pyrroline-N-oxide (DMPO). The reactions of tert-butyl hydroperoxide with Hb and intact cell systems were studied. HbO2 containing system showed exclusive production of the t-butyloxy radical spin adduct of DMPO (DMPO-OBut), indicating t-butyloxy radical production. Methemoglobin-containing systems showed the production of an oxidized derivative of DMPO, 5,5-dimethyl-2-ketopyrrolidino-1-oxyl (DMPOX)-previously associated with the generation of highly oxidized heme-Fe. HbCO containing systems show the production of both DMPO-OBut and DMPOX but markedly slower than in either of the other Hb systems. Generally, free radical production in Hb systems was faster than in intact cell systems, indicating a membrane transport rate-limiting step for the tert-butyl hydroperoxide-mediated effects. Data from the use of free radical scavengers to inhibit DMPO-OBut production was consistent with the known reactivities of the scavengers toward t-butyloxy radicals. Important roles for t-butyloxy radicals and heme-intermediates were implicated in tert-butyl hydroperoxide-induced lipid peroxidation and Hb oxidation in erythrocytes, respectively.