Bgs2p, a 1,3-β-glucan synthase subunit, is essential for maturation of ascospore wall in Schizosaccharomyces pombe
Bgs2p, a 1,3-β-glucan synthase subunit, is essential for maturation of ascospore wall in Schizosaccharomyces pombe
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DOI：
10.1016/s0014-5793(00)01828-7
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发表时间：
2000-07-28
期刊：
影响因子：
3.5
通讯作者：
Balasubramanian, M
中科院分区：
文献类型：
--
作者：
Liu, JH;Tang, X;Balasubramanian, M
以前，我们已经报道，Drc 1 p/Cps 1 p，1，3-β-葡聚糖合酶亚基，是必不可少的分裂隔膜装配粟酒裂殖酵母。在这份报告中，我们提出的证据表明，S。粟酒裂殖酵母Bgs 2 p是一种1，3-β-葡聚糖合成酶，与Drc 1 p/Cps 1 p的同源性为56%。粟酒裂殖酵母，但不需要营养生长。对于bgs 2无效突变纯合的二倍体细胞，以及同宗配合的bgs 2无效突变单倍体正常地进行减数分裂。然而，含有1，3-β-葡聚糖的孢子壁在这些细胞中不组装。由bgs 2缺失突变体减数分裂产生的孢子在从菌丝体释放时裂解，因此是不能存活的。利用绿色荧光蛋白标记的Bgs 2 p，我们证明，Bgs 2 p是本地化在周边的子囊孢子减数分裂和孢子形成。然而，在营养细胞中未检测到Bgs 2 p。我们得出结论，Bgs 2 p是所需的1，3-β-葡聚糖合成过程中子囊孢子壁成熟。(C)2000年欧洲生物化学学会联合会。由Elsevier Science B. V.出版，版权所有。
Previously we have reported that Drc1p/Cps1p, a 1,3-beta-glucan synthase subunit, is essential for division septum assembly in Schizosaccharomyces pombe. In this report, we present evidence that S. pombe Bgs2p, a 1,3-beta-glucan synthase that shows 56% identity to Drc1p/Cps1p, is essential for maturation of ascospore wall in S. pombe but is not required for vegetative growth. Diploid cells homozygous for the bgs2-null mutation, as well as homothallic bgs2-null mutant haploids undergo meiosis normally. However, a 1,3-beta-glucan containing spore wall is not assembled in these cells. The spores resulting from meiosis of a bgs2-null mutant lyse upon release from the ascus and are therefore inviable. Using a green fluorescent protein-tagged Bgs2p, we demonstrate that Bgs2p is localized at the periphery of the ascospore during meiosis and sporulation. However, Bgs2p is not detected in vegetative cells. We conclude that Bgs2p is required for 1,3-beta-glucan synthesis during ascospore wall maturation. (C) 2000 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.