Prebiotic potential of xylooligosaccharides derived from finger millet seed coat
Prebiotic potential of xylooligosaccharides derived from finger millet seed coat
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DOI：
10.1080/08905436.2017.1369433
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发表时间：
2017-01-01
影响因子：
1.8
通讯作者：
Antony, Usha
中科院分区：
文献类型：
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作者：
Palaniappan, Ayyappan;Balasubramaniam, V. Geetha;Antony, Usha
以小米CO 9种皮为原料提取水溶性木聚糖，采用酶法制备低聚木糖，并对其益生潜力进行评价。用热木聚糖酶处理指谷种皮的WSX，XOS的得率为72%。当使用高效液相色谱-蒸发光散射检测器（HPLC-ELSD）分析时获得的XOS指示聚合度（DP）为2，并且电喷雾电离质谱（ESI-MS）揭示木二糖为主要糖。通过傅立叶变换红外光谱（FTIR）和核磁共振（NMR）研究确定了XOS的-糖苷键的存在。XOS具有75%的抗氧化活性和益生元功效。益生菌菌株存在下的益生元活性是菌株特异性的，并且菌株之间的益生元活性评分（PAS）的差异是明显的。与市售XOS相比，在小米种皮XOS上生长的植物乳杆菌无细胞上清液对四种病原微生物表现出较强的抗菌活性。小米种皮代表了提取木聚糖及其用于益生元XOS生产的丰富和替代来源。
The present study deals with the enzymatic production of xylooligosaccharides (XOS) from water-soluble xylan (WSX) extracted from finger millet CO9 seed coat and the evaluation of its prebiotic potential. The yield of XOS was 72%, when the WSX of the finger millet seed coat was treated with commercially available xylanase of Thermomyces lanuginosus. The XOS obtained when analyzed using high-performance liquid chromatography-evaporative light scattering detector (HPLC-ELSD) indicated a degree of polymerization (DP) of 2 and Electro Spray Ionization Mass Spectra (ESI-MS) revealed xylobiose as the major sugar. The presence of -glycosidic linkages of XOS was identified by Fourier Transform Infrared Spectrometer (FTIR) and Nuclear Magnetic Resonance (NMR) studies. The XOS exhibited an antioxidant activity of 75% and prebiotic efficacy. Prebiotic activity in the presence of probiotic strains was strain specific and differences in the prebiotic activity score (PAS) between strains were evident. The cell-free supernatant of Lactobacillus plantarum grown on the XOS from finger millet seed coat showed strong antibacterial activities against four pathogenic microorganisms compared with commercial XOS. Finger millet seed coat represents an abundant and alternative source for the extraction of xylan and its use for prebiotic XOS production.