EFFECT OF COLON FLORA AND SHORT-CHAIN FATTY-ACIDS ON GROWTH IN-VITRO OF PSEUDOMONAS-AERUGINOSA AND ENTEROBACTERIACEAE
EFFECT OF COLON FLORA AND SHORT-CHAIN FATTY-ACIDS ON GROWTH IN-VITRO OF PSEUDOMONAS-AERUGINOSA AND ENTEROBACTERIACEAE
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DOI：
10.1128/iai.8.1.30-35.1973
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发表时间：
1973-01-01
影响因子：
3.1
通讯作者：
LEVISON, ME
中科院分区：
文献类型：
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作者：
LEVISON, ME
以下悬浮液在pH 6.5、6.0和5.5下对铜绿假单胞菌具有热稳定抗菌活性：（i）正常小鼠的合并结肠内容物;（ii）正常小鼠粪便的厌氧48小时培养物;和（iii）来自人类结肠植物群的不同细菌（大肠杆菌、脆弱拟杆菌、肺炎克雷伯氏菌和奇异变形杆菌）的厌氧48小时培养物。培养基的pH值越低，这些悬浮液的抗菌活性越大。5种脂肪酸（丙酸、丁酸、异丁酸、乙酸和甲酸）的抑菌活性对P.对三种肠杆菌科（E. coli，K. pneumoniae和P.在所有脂肪酸浓度（0.16 M至0.005 M）和研究的3个pH值（5.5、6.0和6.5）下，随着pH值的升高，抗菌活性降低。在较高的脂肪酸浓度下，抗菌活性更大，并且在每个pH值下，具有高pKa值的脂肪酸的抗菌活性最大。具有最低pKa的乳酸表现出很少或没有抗菌活性。乙酸和丁酸，两个三个主要的挥发性脂肪酸测定气相色谱法在小鼠结肠内容物和小鼠粪便的厌氧培养，发生在体内的浓度，抑制生长ofP。在小鼠盲肠的pH值下，在体外对产气荚膜梭菌进行培养。这些结果表明，由结肠植物群产生的未解离的短链脂肪酸可能是肠道抵抗P.铜绿。
Heat-stable antibacterial activity in the following suspensions was demonstrated againstPseudomonas aeruginosaat pH 6.5, 6.0, and 5.5: (i) pooled colon contents of normal mice; (ii) an anaerobic, 48-h culture of normal mouse feces; and (iii) anaerobic, 48-h cultures of different bacteria from human colon flora (Escherichia coli, Bacteroides fragilis, Klebsiella pneumoniae, andProteus mirabilis). The lower the pH of the medium, the greater was the antibacterial activity of these suspensions. The antibacterial activity of five fatty acids (propionic, butyric, isobutyric, acetic, and formic acids) was greater againstP. aeruginosathan against threeEnterobacteriaceae(E. coli, K. pneumoniae, andP. mirabilis) at all fatty acid concentrations (0.16 M to 0.005 M) and at the 3 pH values studied (5.5, 6.0, and 6.5). As the pH value increased, the antibacterial activity decreased. Antibacterial activity was greater at higher fatty acid concentrations, and at each pH value it was greatest for the fatty acids having high pKavalues. Lactic acid, with the lowest pKa, exhibited little or no antibacterial activity. Acetic and butyric acids, two of the three predominant volatile fatty acids determined by gas chromatography in the mouse colon contents and in the anaerobic culture of mouse feces, occurred in vivo in concentrations which inhibited growth ofP. aeruginosain vitro at the pH of the mouse cecum. These results suggest that undissociated short-chain fatty acids produced by the colon flora may be a mechanism of intestinal resistance to colonization byP. aeruginosa.