MRI Measurement of Regional Lung Deposition in Mice Exposed Nose-Only to Nebulized Superparamagnetic Iron Oxide Nanoparticles
MRI Measurement of Regional Lung Deposition in Mice Exposed Nose-Only to Nebulized Superparamagnetic Iron Oxide Nanoparticles
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DOI：
10.1089/jamp.2008.0698
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发表时间：
2008-12-01
影响因子：
3.4
通讯作者：
Finlay, Warren H.
中科院分区：
文献类型：
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作者：
Martin, Andrew R.;Thompson, Richard B.;Finlay, Warren H.
超顺磁性氧化铁纳米颗粒显示出将吸入的气溶胶磁性靶向至肺部局部部位的潜力。这些颗粒也用作磁共振成像（MRI）中的造影剂。在目前的工作中，我们研究的可行性，测量区域肺沉积的氧化铁纳米粒子使用MRI。将小鼠仅暴露于雾化的超顺磁性氧化铁纳米颗粒。使用飞行时间装置测量吸入室中的液滴尺寸分布。与基线组相比，通过测量暴露小鼠肺组织的纵向弛豫时间（T-1），用MRI评估左肺和右肺中铁的区域浓度。小鼠肺中的局部铁浓度范围为1.1 +/- 0.8 μ g/cm（3）（平均值+/-一个标准差，n = 6）（外周肺区域）至2.7 +/- 1.4 μ g/cm（3）（中央肺区域），左右肺之间无显著差异。吸入室中雾化液滴的质量中值空气动力学直径（MMAD）为5.6 +/-08 μ m，几何标准偏差（GSD）为1.30 +/-0.03（两个值均表示为平均值+/-1个标准偏差，n = 6）。MRI显示出在体内测量超顺磁性氧化铁纳米颗粒的局部肺浓度的前景，并且可能在肺沉积和清除的研究中有用。
Superparamagnetic iron oxide nanoparticles show potential in magnetic targeting of inhaled aerosols to localized sites within the lung. These particles are also used as contrast agents in magnetic resonance imaging (MRI). In the present work, we examine the feasibility of measuring regional lung deposition of iron oxide nanoparticles using MRI. Mice were exposed nose-only to nebulized superparamagnetic iron oxide nanoparticles. The droplet size distribution in the inhalation chamber was measured using a time-of-flight device. Regional concentrations of iron in the left and right lung were assessed with MRI by measuring the longitudinal relaxation times (T-1) of the lung tissue in exposed mice, compared to a baseline group. Regional concentrations of iron in the lungs of the mice ranged from 1.1 +/- 0.8 mu g/cm(3) (mean +/- one standard deviation, n = 6) in peripheral lung regions to 2.7 +/- 1.4 mu g/cm(3) in the central lung, with no significant difference between the left and right lung. The nebulized droplets in the inhalation chamber had mass median aerodynamic diameter (MMAD) of 5.6 +/- 08 mu m, with a geometric standard deviation (GSD) of 1.30 +/- 0.03 (both values expressed as mean +/- one standard deviation, n = 6). MRI shows promise for in vivo measurement of regional lung concentrations Of Superparamagnetic iron oxide nanoparticles, and may be useful in studies of lung deposition and clearance.