Calcium wave propagation in networks of endothelial cells: model-based theoretical and experimental study.
Calcium wave propagation in networks of endothelial cells: model-based theoretical and experimental study.
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DOI：
10.1371/journal.pcbi.1002847
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发表时间：
2012
影响因子：
4.3
通讯作者：
Deymier P
中科院分区：
文献类型：
--
作者：
Long J;Junkin M;Wong PK;Hoying J;Deymier P
在本文中，我们对由人内皮细胞组成的多细胞结构中钙信号的传播进行了理论和实验相结合的研究。我们考虑由单细胞链以及具有侧分支的细胞链组成的多细胞结构，即“T”结构。在实验中，我们研究了应用机械刺激沿链诱导钙波形式信号传导的结果，以及单次和双重刺激对多细胞结构的影响。实验结果提供了建筑对钙波传播影响的证据。基于钙诱导的钙释放和通过间隙连接的细胞间扩散模型的模拟表明，钙波的传播取决于细胞内钙调节和结构依赖性细胞间扩散之间的竞争。在多种细胞类型中都发现了钙波信号。近年来大量的钙实验表明，钙信号不仅是细胞内的调节因子，而且能够作为细胞间信号传递到周围细胞。本文重点开发一种理论和实验方法互补整合的方法，用于研究多细胞结构中的多级相互作用及其对集体细胞动态行为的影响。我们描述了由低阶（细胞内）现象引起的新型高阶（跨结构）行为，并对多细胞生物系统动力学的潜在机制进行预测。该理论方法以数字方式描述了细胞模型网络中基于钙的信号传导的非线性行为的动态。人脐静脉内皮细胞（HUVEC）的微工程几何约束网络作为平台，根据网络拓扑对新兴钙信号时空特征的影响来仲裁理论预测。
In this paper, we present a combined theoretical and experimental study of the propagation of calcium signals in multicellular structures composed of human endothelial cells. We consider multicellular structures composed of a single chain of cells as well as a chain of cells with a side branch, namely a “T” structure. In the experiments, we investigate the result of applying mechano-stimulation to induce signaling in the form of calcium waves along the chain and the effect of single and dual stimulation of the multicellular structure. The experimental results provide evidence of an effect of architecture on the propagation of calcium waves. Simulations based on a model of calcium-induced calcium release and cell-to-cell diffusion through gap junctions shows that the propagation of calcium waves is dependent upon the competition between intracellular calcium regulation and architecture-dependent intercellular diffusion. Calcium wave signal has been found in a wide variety of cell types. Over the last years, a large number of calcium experiments have shown that calcium signal is not only an intracellular regulator but is also able to be transmitted to surrounding cells as intercellular signal. This paper focuses on the development of an approach with complementary integration of theoretical and experimental methods for studying the multi-level interactions in multicellular architectures and their effect on collective cell dynamic behavior. We describe new types of higher-order (across structure) behaviors arising from lower-order (within cells) phenomena, and make predictions concerning the mechanisms underlying the dynamics of multicellular biological systems. The theoretical approach describes numerically the dynamics of non-linear behavior of calcium-based signaling in model networks of cells. Microengineered, geometrically constrained networks of human umbilical vein endothelial cells (HUVEC) serve as platforms to arbitrate the theoretical predictions in terms of the effect of network topology on the spatiotemporal characteristics of emerging calcium signals.
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影响因子：
2.4
作者：
Deymier, Pierre A.;Runge, Keith;Vasseur, Jerome O.
通讯作者：
Vasseur, Jerome O.
影响因子：
56.9
作者：
FINCH, EA;TURNER, TJ;GOLDIN, SM
通讯作者：
GOLDIN, SM
DOI：
10.1152/ajpheart.00425.2011
发表时间：
2011-09-01
影响因子：
4.8
作者：
Bagher, Pooneh;Davis, Michael J.;Segal, Steven S.
通讯作者：
Segal, Steven S.
DOI：
10.1161/01.atv.11.5.1258
发表时间：
1991-09-01
期刊：
ARTERIOSCLEROSIS AND THROMBOSIS
影响因子：
--
作者：
DRUMHELLER, PD;HUBBELL, JA
通讯作者：
HUBBELL, JA
影响因子：
4
作者：
Junkin, Michael;Leung, Siu Ling;Wong, Pak Kin
通讯作者：
Wong, Pak Kin