PGE(2) inhibits glucose uptake in isolated villous epithelial cells of the ovine small intestine
PGE(2) inhibits glucose uptake in isolated villous epithelial cells of the ovine small intestine
复制标题
DOI：
10.1111/j.1439-0442.1997.tb01129.x
复制
发表时间：
1997-09-01
期刊：
影响因子：
--
通讯作者：
Kato, S
中科院分区：
文献类型：
--
作者：
Hyun, HS;Arai, S;Kato, S
本研究旨在阐明PGE(2)是否直接抑制绒毛细胞对葡萄糖的吸收。为此，我们使用分离的绵羊小肠绒毛上皮细胞，在0.2 mM苯丙素或10 μ M PGE存在和不存在的情况下，测量吸收上皮细胞对c -14标记的葡萄糖的摄取(2)。在绵羊离体小肠绒毛上皮细胞中，根霉素的存在使净葡萄糖吸收率降低了68.2%。这一结果表明，反刍动物体内的大部分葡萄糖是由一种特异性转运体SGLT1吸收的，该转运体对苯并菌素很敏感。PGE(2)使绵羊离体绒毛上皮细胞对葡萄糖的吸收降低了26.5%，尽管这种对葡萄糖的吸收在苯丙素的存在下没有改变。这一结果清楚地表明，PGE(2)通过直接作用于反刍动物的绒毛细胞，抑制了苯酞敏感的葡萄糖吸收过程。
This study was designed to clarify whether PGE(2) directly inhibits glucose absorption in villous cells. For this purpose, isolated villous epithelial cells of the ovine small intestine were used, and the uptake of C-14-labelled glucose in the absorptive epithelial cells was measured in the presence and absence of 0.2 mM phlorizin or 10 mu M PGE(2). In isolated villous epithelial cells of the small intestine in sheep, net glucose absorption was reduced by 68.2% in the presence of phlorizin. This result indicates that most of the glucose in ruminants is absorbed by the specific transporter SGLT1, which is sensitive to phlorizin. PGE(2) decreased glucose absorption by 26.5% in isolated villous epithelial cells in sheep, although this glucose absorption did not change in the presence of phlorizin. This result clearly indicates the PGE(2) inhibits the phlorizin-sensitive glucose absorptive process through direct action on the villous cells of ruminants.