Association between anti-Pfs48/45 reactivity and P-falciparum transmission-blocking activity in sera from Cameroon
Association between anti-Pfs48/45 reactivity and P-falciparum transmission-blocking activity in sera from Cameroon
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DOI：
10.1046/j.1365-3024.1996.d01-54.x
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发表时间：
1996-02-01
影响因子：
2.2
通讯作者：
Sauerwein, R
中科院分区：
文献类型：
--
作者：
Roeffen, W;Mulder, B;Sauerwein, R
Pfs 48/45是恶性疟原虫的有性阶段寄生虫蛋白双联体，是抑制寄生虫在蚊子中发育的抗体的靶标。28（54%）的52血清配子体载体从喀麦隆减少感染性的蚊子膜喂养生物测定不到20%的控制。通过竞争ELISA分析这52份血清中是否存在能够与针对Pfs 48/45上5个不同表位的6种单克隆抗体（MoAb）结合的抗体。这52个喀麦隆血清与单克隆抗体之一竞争的百分比范围从13%（表位I）到33%（表位IIc）。在传递阻断试验（大于或等于80%）和Pfs 48/45竞争ELISA中的活性比较显示相对特异性为100%（24/24），相对灵敏度为75%（21/28）。非阻断血清显示与任何单克隆抗体没有竞争。这些单克隆抗体进一步用于研究表位的多样性之间的恶性疟原虫分离株使用双位点ELISA。针对表位I、III和V的单克隆抗体与四种不同的分离株反应，而表位II可以细分为三个表位。没有分离物与MoAb 3G 12（表位IV）反应。使用这四种不同的分离株，竞争ELISA滴度从1/20变化到1/80，并且除了表位II之外，在分离株之间没有发现显著差异，其中在11个表位IIa阳性的分离株中只有3个也是表位IIc阳性的。
Pfs48/45, a sexual stage parasite protein doublet of P. falciparum, is a target of antibodies which inhibit the development of the parasite in the mosquito. Twenty-eight (54%) out of 52 sera of gametocyte carriers from Cameroon reduced infectivity in the mosquito membrane feeding bioassay to less than 20% of the controls. These 52 sera were analysed by competition ELISAs for the presence of antibodies capable of compering the binding of six monoclonal antibodies (MoAbs) directed against five different epitopes on Pfs48/45. The percentage of these 52 Cameroon sera that competed with one of the MoAbs ranged from 13% (epitope I) to 33% (epitope IIc). Comparison of activity in the transmission-blocking assay (greater than or equal to 80%) and in the Pfs48/45 competition ELISA show a relative specificity of 100% (24 of 24) and a relative sensitivity, of 75% (21 of 28). Non-blocking sera showed no competition with any of the MoAbs. These MoAbs were further used to study the diversity of epitopes among isolates of P. falciparum using a two-site ELISA. MoAbs against epitope I, III and V reacted with four different isolates whereas epitope II could be subdivided into three epitopes. None of the isolates reacted with MoAb 3G12 (epitope IV). Using these four different isolates, the competition ELISA titre varies from 1/20 to 1/80 and no significant differences are found between the isolates except for epitope II where only three out of 11 positives for epitope IIa were also positive for epitope IIc.