Prevalence of eae-positive, lactose non-fermenting Escherichia albertii from retail raw meat in China
Prevalence of eae-positive, lactose non-fermenting Escherichia albertii from retail raw meat in China
复制标题
中国零售生肉中 EAE 阳性、乳糖非发酵埃希氏菌的流行情况
DOI：
10.1017/s0950268815001120
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发表时间：
2016-01-01
影响因子：
4.2
通讯作者：
Xiong, Y.
中科院分区：
文献类型：
--
作者：
Wang, H.;Li, Q.;Xiong, Y.
摘要 阿尔伯特埃希菌是一种新出现的肠道病原体，与人类胃肠炎有关。最近，在健康和患病的鸟类、动物、鸡肉和水中也检测到了艾伯特艾伯球菌。在本研究中，评估了中国零售生肉中 EAE 阳性、乳糖非发酵艾伯特艾氏菌菌株的流行情况和特征。从446份（6·73%）样品中分离出30株艾伯氏埃博拉菌，包括鸭肠（21·43%，6/28）、鸭肉（9·52%，2/21）、鸡肠（8·99%，17/189）、鸡肉（5·66%，3/53）、羊肉（4·55%，4/55%）。 1/22) 和猪肉(2·44%, 1/41)。从 92 个生牛肉样品中没有分离出任何一种。通过表型特性、诊断性 PCR、16S rRNA 基因和管家基因的序列分析，菌株被鉴定为 E. albertii。这些菌株含有五种 intimin 亚型。所有菌株都具有 cdtB 基因的 II/III/V 亚型组，其中两个菌株携带 I/IV 亚型组的另一个拷贝。脉冲场凝胶电泳显示生肉中艾伯氏菌具有高度遗传多样性。我们的研究结果表明，艾伯氏菌可以污染各种生肉，对公众健康构成潜在威胁。
SUMMARY Escherichia albertii is a newly emerging enteric pathogen that has been associated with gastroenteritis in humans. Recently, E. albertii has also been detected in healthy and sick birds, animals, chicken meat and water. In the present study, the prevalence and characteristics of the eae-positive, lactose non-fermenting E. albertii strains in retail raw meat in China were evaluated. Thirty isolates of such strains of E. albertii were identified from 446 (6·73%) samples, including duck intestines (21·43%, 6/28), duck meat (9·52%, 2/21), chicken intestines (8·99%, 17/189), chicken meat (5·66%, 3/53), mutton meat (4·55%, 1/22) and pork meat (2·44%, 1/41). None was isolated from 92 samples of raw beef meat. Strains were identified as E. albertii by phenotypic properties, diagnostic PCR, sequence analysis of the 16S rRNA gene, and housekeeping genes. Five intimin subtypes were harboured by these strains. All strains possessed the II/III/V subtype group of the cdtB gene, with two strains carrying another copy of the I/IV subtype group. Pulsed-field gel electrophoresis showed high genetic diversity of E. albertii in raw meats. Our findings indicate that E. albertii can contaminate various raw meats, posing a potential threat to public health.