Trehalose-enhanced isolation of neuronal sub-types from adult mouse brain.
Trehalose-enhanced isolation of neuronal sub-types from adult mouse brain.
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DOI：
10.2144/0000113878
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发表时间：
2012-06
期刊：
影响因子：
2.7
通讯作者：
Carninci P
中科院分区：
文献类型：
--
作者：
Saxena A;Wagatsuma A;Noro Y;Kuji T;Asaka-Oba A;Watahiki A;Gurnot C;Fagiolini M;Hensch TK;Carninci P
由于巩固神经元网络的细胞外基质的复杂性，从成人大脑中有效地分离特定的、完整的、活的神经元是有问题的。在这里，我们提出了使用荧光激活细胞分选(FACS)从成熟的小鼠脑中分离纯神经元群体的方案的重大改进。细胞产量增加了10倍，使得通过纳米笼子和RNA序列等协议进行细胞特异性转录组分析成为可能。
Efficient isolation of specific, intact, living neurons from the adult brain is problematic due to the complex nature of the extracellular matrix consolidating the neuronal network. Here, we present significant improvements to the protocol for isolation of pure populations of neurons from mature postnatal mouse brain using fluorescence activated cell sorting (FACS). The 10-fold increase in cell yield enables cell-specific transcriptome analysis by protocols such as nano-CAGE and RNA seq.