The Dynamic Changes of the Plasma Membrane Proteins and the Protective Roles of Nitric Oxide in Rice Subjected to Heavy Metal Cadmium Stress.
The Dynamic Changes of the Plasma Membrane Proteins and the Protective Roles of Nitric Oxide in Rice Subjected to Heavy Metal Cadmium Stress.
复制标题
重金属镉胁迫下水稻质膜蛋白的动态变化及一氧化氮的保护作用
DOI：
10.3389/fpls.2016.00190
复制
发表时间：
2016
影响因子：
5.6
通讯作者：
Hu X
中科院分区：
文献类型：
--
作者：
Yang L;Ji J;Harris-Shultz KR;Wang H;Wang H;Abd-Allah EF;Luo Y;Hu X
重金属镉是土壤中常见的环境污染物，对农作物生长发育有不良影响。在植物中，启动细胞对环境胁迫的反应的信号传导过程已被证明位于质膜（PM）中。更好地了解PM蛋白质组对环境胁迫的响应可能会为改善耐逆作物提供新的见解。一氧化氮（NO）参与了植物对镉胁迫的响应。为了进一步研究在镉胁迫过程中，NO如何调节质膜蛋白质的变化，定量蛋白质组学方法的基础上同量异位素标签的相对和绝对定量（iTRAQ）被用来确定差异调节的蛋白质从水稻质膜镉或镉和NO处理后。共鉴定出66个差异表达蛋白，其中许多蛋白具有转运蛋白、ATP酶、激酶、代谢酶、磷酸酶和磷脂酶的功能。其中，磷脂酶D（PLD）的丰度在镉或镉和NO处理后发生了显着变化。PLD融合绿色荧光肽（GFP）在水稻原生质体中的瞬时表达表明，镉和NO处理促进PLD在质膜上的积累。NO的加入也增强了Cd诱导的PLD活性和PLD活性产生的磷脂酸（PA）的积累。同时，NO还能提高抗氧化酶的活性，促进谷胱甘肽的积累，从而降低Cd诱导的H2O2积累。综上所述，我们认为NO信号与抗氧化酶、谷胱甘肽和PA的积累有关，这些抗氧化酶通过抗氧化防御系统提高水稻对镉的耐受性。
The heavy metal cadmium is a common environmental contaminant in soils and has adverse effects on crop growth and development. The signaling processes in plants that initiate cellular responses to environmental stress have been shown to be located in the plasma membrane (PM). A better understanding of the PM proteome in response to environmental stress might provide new insights for improving stress-tolerant crops. Nitric oxide (NO) is reported to be involved in the plant response to cadmium (Cd) stress. To further investigate how NO modulates protein changes in the plasma membrane during Cd stress, a quantitative proteomics approach based on isobaric tags for relative and absolute quantification (iTRAQ) was used to identify differentially regulated proteins from the rice plasma membrane after Cd or Cd and NO treatment. Sixty-six differentially expressed proteins were identified, of which, many function as transporters, ATPases, kinases, metabolic enzymes, phosphatases, and phospholipases. Among these, the abundance of phospholipase D (PLD) was altered substantially after the treatment of Cd or Cd and NO. Transient expression of the PLD fused with green fluorescent peptide (GFP) in rice protoplasts showed that the Cd and NO treatment promoted the accumulation of PLD in the plasma membrane. Addition of NO also enhanced Cd-induced PLD activity and the accumulation of phosphatidic acid (PA) produced through PLD activity. Meanwhile, NO elevated the activities of antioxidant enzymes and caused the accumulation of glutathione, both which function to reduce Cd-induced H2O2 accumulation. Taken together, we suggest that NO signaling is associated with the accumulation of antioxidant enzymes, glutathione and PA which increases cadmium tolerance in rice via the antioxidant defense system.