High activity of K+-dependent plasmalemmal Na+/Ca2+ exchangers in hippocampal CA1 neurons.
High activity of K+-dependent plasmalemmal Na+/Ca2+ exchangers in hippocampal CA1 neurons.
复制标题
海马 CA1 神经元中 K 依赖性质膜 Na /Ca2 交换器的高活性。
DOI：
10.1097/00001756-200409150-00023
复制
发表时间：
2004
期刊：
影响因子：
1.7
通讯作者：
Kiedrowski,Lech
中科院分区：
文献类型：
--
作者：
Kiedrowski,Lech
全脑缺血后，Ca 2+通过依赖性和非依赖性Na+/Ca 2+交换体（NCKX和NCX）内流，在神经元死亡中起重要作用，其中CA 1区神经元尤为脆弱。因此，本研究探讨了在体外培养的大鼠海马CA 1区神经元中，NCKX和NCX的Ca 2+内流速率是否与前脑神经元（FN）和小脑颗粒细胞（CGC）的Ca 2+内流速率不同。NCKX介导的Ca 2+内流在CA 1神经元中比在FN或CGCs中快几倍，并且不受Na+/Ca 2+交换抑制剂KB-R7943或苄普地尔的影响。NCKX逆转抑制剂尚未上市。它们的开发将极大地促进进一步测试NCKX在CA 1神经元缺血性死亡中的作用。
Ca 2+ influx via reversed K+-dependent (NCKX) and/or K+-independent (NCX) plasmalemmal Na+/Ca 2+ exchangers may play a role in neuronal death following global brain ischemia to which CA1 neurons are particularly vulnerable. Therefore, this work tested whether the rates of Ca 2+ influx via reversed NCKX or NCX in cultured rat CA1 neurons differ from those in forebrain neurons (FNs) or cerebellar granule cells (CGCs). The NCKX-mediated Ca 2+ influx was several times more rapid in CA1 neurons than in FNs or CGCs and was not affected by Na+/Ca 2+ exchange inhibitors, KB-R7943 or bepridil. NCKX reversal inhibitors are not yet available. Their development would greatly facilitate further testing the role of NCKX in ischemic death of CA1 neurons.