Seawater acidification induced immune function changes of haemocytes in Mytilus edulis: a comparative study of CO(2) and HCl enrichment.
Seawater acidification induced immune function changes of haemocytes in Mytilus edulis: a comparative study of CO(2) and HCl enrichment.
复制标题
海水酸化诱导贻贝血细胞免疫功能变化：CO2和HCl富集的对比研究
DOI：
10.1038/srep41488
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发表时间：
2017-02-06
影响因子：
4.6
通讯作者：
Wang Y
中科院分区：
文献类型：
--
作者：
Sun T;Tang X;Jiang Y;Wang Y
本研究旨在评价CO2-或HCl诱导的海水酸化（pH 7.7或7.1;对照：pH 8.1）对紫贻贝血细胞的影响，并在21 d的实验期间研究其结构和免疫功能的变化。结果表明，海水酸化对细胞死亡率和粒细胞比例影响不大，但对粒细胞超微结构有一定的破坏作用。血细胞的吞噬作用也以明显的浓度依赖性方式受到显著抑制，表明免疫功能受到影响。此外，CO2和HCl处理均显著诱导了ROS的产生，GSH、GST、GR和GPx 4种抗氧化成分对酸化胁迫有积极的响应。相比之下，在相同的pH值水平下，CO2对血细胞的破坏作用比HCl更严重，表明CO2以其他方式胁迫细胞，超出了增加H+浓度。一种可能的解释是海水酸化导致ROS过度产生，破坏了血细胞的超微结构，降低了吞噬作用。
The present study was performed to evaluate the effects of CO2− or HCl-induced seawater acidification (pH 7.7 or 7.1; control: pH 8.1) on haemocytes of Mytilus edulis, and the changes in the structure and immune function were investigated during a 21-day experiment. The results demonstrated that seawater acidification had little effect on the cellular mortality and granulocyte proportion but damaged the granulocyte ultrastructure. Phagocytosis of haemocytes was also significantly inhibited in a clearly concentration-dependent manner, demonstrating that the immune function was affected. Moreover, ROS production was significantly induced in both CO2 and HCl treatments, and four antioxidant components, GSH, GST, GR and GPx, had active responses to the acidification stress. Comparatively, CO2 had more severe destructive effects on haemocytes than HCl at the same pH level, indicating that CO2 stressed cells in other ways beyond the increasing H+ concentration. One possible explanation was that seawater acidification induced ROS overproduction, which damaged the ultrastructure of haemocytes and decreased phagocytosis.