Gene expression profiling of human placentas from preeclamptic and normotensive pregnancies
Gene expression profiling of human placentas from preeclamptic and normotensive pregnancies
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DOI：
10.1093/molehr/gal011
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发表时间：
2006-03-01
影响因子：
4
通讯作者：
Brownstein, MJ
中科院分区：
文献类型：
--
作者：
Hansson, SR;Chen, Y;Brownstein, MJ
本研究的目的是调查先兆子痫（PE）患者，持续性双侧子宫动脉切迹（无PE）和正常对照组胎盘样本中的基因表达模式。这项研究包括胎盘组织从9名妇女与PE，7个简单的妊娠和5个双侧子宫动脉切迹多普勒测速描记。使用具有6500个转录物/基因的人cDNA微阵列，并通过实时PCR和原位杂交验证结果。多维标度法和随机排列法显示三组间差异有统计学意义。在6.5K阵列中，6198个元件是独特的cDNA克隆，代表5952个独特的UniGenes和5695个独特的LocusLinks。多维标度图显示5000个基因符合我们的质量标准，其中366个基因在至少一次比较中有显著差异。实时PCR和原位杂交证实了三个感兴趣的基因的差异;酸性磷酸酶5在PE样本中过表达，钙调蛋白2和v-rel网状内皮组织增生症病毒癌基因同源物A（RELA）在PE和子宫动脉切迹胎盘中下调。总之，RELA和钙调素2的下调可能代表胎盘试图补偿细胞内钙的升高，可能是由缺氧和/或细胞凋亡引起的，在子宫动脉切迹和先兆子痫的妊娠中。
The aim of this study was to investigate patterns of gene expression in placental samples from patients with preeclampsia (PE), persistent bilateral uterine artery notching (without PE), and normal controls. This study included placental tissue from nine women with PE, seven with uncomplicated pregnancies and five with bilateral uterine artery notching in Doppler velocimetry tracings. Human cDNA microarrays with 6500 transcripts/genes were used and the results verified with real-time PCR and in-situ hybridization. Multidimensional scaling method and random permutation technique demonstrated significant differences among the three groups examined. Within the 6.5K arrays, 6198 elements were unique cDNA clones representing 5952 unique UniGenes and 5695 unique LocusLinks. Multidimensional scaling plots showed 5000 genes that met our quality criteria; among these, 366 genes were significantly different in at least one comparison. Differences in three genes of interest were confirmed with real-time PCR and in-situ hybridization; acid phosphatase 5 was shown to be overexpressed in PE samples and calmodulin 2 and v-rel reticuloendotheliosis viral oncogene homolog A (RELA) were downregulated in PE and uterine artery notch placentas. In conclusion downregulation of RELA and calmodulin 2 might represent an attempt by the placenta to compensate for elevations in intracellular calcium, possibly caused by hypoxia and/or apoptosis, in both pregnancies with uterine artery notching and preeclampsia.