Synthesis of photolabile transcription initiators and preparation of photocleavable functional RNA by transcription
Synthesis of photolabile transcription initiators and preparation of photocleavable functional RNA by transcription
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DOI：
10.1016/j.bmcl.2012.05.028
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发表时间：
2012-07-01
影响因子：
2.7
通讯作者：
Shi, Yongliang
中科院分区：
文献类型：
--
作者：
Huang, Faqing;Shi, Yongliang
化学合成了两种具有末端硫醇和氨基官能团的新型光敏腺苷转录启动子。在T7 phi2.5启动子下的转录启动子存在下的转录产生50个巯基和氨基功能化的RNA，由光可切割（PC）连接子偶联。生物素进一步的RNA功能化可以通过从生物素基AMP到RNA巯基或生物素NHS到RNA氨基的酰基转移反应来实现。PC接头的光裂解反应速度相对较快，半衰期为4-5分钟。这些转录启动子的可用性使得新的可光性RNA可用于RNA的亲和纯化、功能性RNA的体外选择和功能性RNA的笼化。(C) 2012 Elsevier Ltd.版权所有。
Two new photolabile adenosine-containing transcription initiators with terminal thiol and amino functionalities are chemically synthesized. Transcription in the presence of the transcription initiators under the T7 phi2.5 promoter produces 50 thiol- and amino-functionalized RNA conjugated by a photocleavable (PC) linker. Further RNA functionalization with biotin may be achieved through acyl transfer reactions from either biotinyl AMP to the RNA thiol group or biotin NHS to the RNA amino group. Photocleavage of the PC linker displays relatively fast kinetics with a half-life of 4-5 min. The availability of these transcription initiators makes new photolabile RNA accessible for affinity purification of RNA, in vitro selection of functional RNAs, and functional RNA caging. (C) 2012 Elsevier Ltd. All rights reserved.