Movement of replicating DNA through a stationary replisome
Movement of replicating DNA through a stationary replisome
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DOI：
10.1016/s1097-2765(00)00130-1
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发表时间：
2000-12-01
期刊：
影响因子：
16
通讯作者：
Grossman, AD
中科院分区：
文献类型：
--
作者：
Lemon, KP;Grossman, AD
我们发现DNA是在一个中心固定聚合酶上复制的，每个复制区域都从复制体上移开。在枯草芽孢杆菌中，DNA聚合酶主要位于或靠近中细胞。当复制在特定的染色体区域被阻断时，该区域位于DNA聚合酶的中心。释放阻滞后，复制区域的每个拷贝都位于相反的细胞两极，远离中央复制体。在大致同步的细胞群中，起点和终点之间的染色体区域在复制之前移动到复制体。因此，复制叉上的聚合酶是固定的，模板在复制过程中被拉入并向外释放。我们提出枯草芽孢杆菌，可能还有许多细菌，利用一个固定的复制体在核苷酸凝聚过程中释放的能量来促进染色体的分裂。
We found that DNA is replicated at a central stationary polymerase, and each replicated region moves away from the replisome. In Bacillus subtilis, DNA polymerase is predominantly located at or near midcell. When replication was blocked in a specific chromosomal region, that region was centrally located with DNA polymerase. Upon release of the block, each copy of the duplicated region was located toward opposite cell poles, away from the central replisome. In a roughly synchronous population of cells, a region of chromosome between origin and terminus moved to the replisome prior to duplication. Thus, the polymerase at the replication forks is stationary, and the template is pulled in and released outward during duplication. We propose that a. subtilis, and probably many bacteria, harness energy released during nucleotide condensation by a stationary replisome to facilitate chromosome partitioning.