Fluorescence-lifetime-based tomography for turbid media
Fluorescence-lifetime-based tomography for turbid media
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DOI：
10.1364/ol.30.003347
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发表时间：
2005-12-15
期刊：
影响因子：
3.6
通讯作者：
Dunn, AK
中科院分区：
文献类型：
--
作者：
Kumar, ATN;Skoch, J;Dunn, AK
收到2005年6月27日;修订稿收到2005年8月17日;接受2005年8月18日我们推导出一种新的算法来恢复在体内分布的荧光团的基础上的渐近寿命分析的时域荧光测量混浊组织。我们通过实验证明了这种方法在定位具有不同寿命的荧光团方面所提供的优势。该算法具有广泛的适用性诊断荧光成像在几厘米厚的生物组织的存在下，因为荧光寿命是一个敏感的指标，局部组织环境和相互作用的分子水平。(c)2005年，美国光学学会。
Received June 27, 2005; revised manuscript received August 17, 2005; accepted August 18, 2005 We derive a novel algorithm to recover the in vivo distributions of fluorophores based on an asymptotic lifetime analysis of time-domain fluorescence measurements with turbid tissue. We experimentally demonstrate the advantage offered by this method in localizing fluorophores with distinct lifetimes. This algorithm has wide applicability for diagnostic fluorescence imaging in the presence of several-centimeter-thick biological tissue, since fluorescence lifetime is a sensitive indicator of local tissue environment and interactions at the molecular level. (c) 2005 Optical Society of America.