Hyperglycemic brain injury in the rat
Hyperglycemic brain injury in the rat
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DOI：
10.1016/j.brainres.2005.12.072
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发表时间：
2006-03-03
期刊：
影响因子：
2.9
通讯作者：
Saporta, S
中科院分区：
文献类型：
--
作者：
Malone, JI;Hanna, SK;Saporta, S
5岁前患糖尿病的儿童神经认知功能下降。这个问题归因于低血糖，胰岛素治疗的并发症。强化胰岛素治疗可减少糖尿病的眼睛、肾脏和神经并发症（高血糖），但与严重低血糖增加3倍相关，因此不建议13岁以下儿童使用。由于在儿童早期糖尿病中高血糖比间歇性低血糖更常见，因此确定高血糖是否影响大脑生长和发育是很重要的。从4周龄到8周龄，大鼠暴露于连续4周的高血糖（糖尿病）或间歇性（3小时，3次/周）低血糖。这些动物的大脑与年龄相仿的正常对照动物的大脑进行了比较。以DNA/脑湿重和蛋白质/DNA含量测定，糖尿病动物皮质细胞数量增加，细胞大小减小。蛋白质、脂肪酸和胆固醇/克DNA的减少也表明，糖尿病动物皮层的髓磷脂含量减少，细胞变小。这些大脑的组织学评估证实了生化结果。这些观察结果需要进一步确认和评估，但表明持续高血糖可能比间歇性低血糖对发育中的大脑更有害。这是治疗幼儿糖尿病的一个重要考虑因素。(c) 2005 Elsevier B.V.版权所有
Children with diabetes onset before 5 years of age have reduced neurocognitive function. This problem has been attributed to hypoglycemia, a complication of insulin therapy. The eye, kidney, and nerve complications of diabetes (hyperglycemia) have been reduced by intensified insulin therapy which is associated with a 3-fold increase in severe hypoglycemia and therefore is not recommended for children less than 13 years of age. Since hyperglycemia is much more common than intermittent hypoglycemia during early childhood diabetes, it is important to determine if hyperglycemia affects brain growth and development. Rats were exposed to 4 weeks of either continuous hyperglycemia (diabetes) or intermittent (3 h, 3 times/week) hypoglycemia from 4 to 8 weeks of age. The brains of these animals were compared to those of similarly aged normal control animals. The cell number was increased, and the cell size reduced in the cortex of diabetic animals as assessed by DNA/wet weight of brain and protein/DNA content. Reduced amounts of protein, fatty acids, and cholesterol/mu gram DNA also indicate smaller cells with reduced myelin content in the cortex of the diabetic animals. Histologic evaluation of these brains confirmed the biochemical findings. These observations require further confirmation and evaluation but indicate that continuous hyperglycemia may be more damaging than intermittent hypoglycemia to the developing brain. This is an important consideration for the management of diabetes mellitus in young children. (c) 2005 Elsevier B.V. All rights reserved.