Correlation between oxytocin neuronal sensitivity and oxytocin receptor binding: an electrophysiological and autoradiographical study comparing rat and guinea pig hippocampus.
Correlation between oxytocin neuronal sensitivity and oxytocin receptor binding: an electrophysiological and autoradiographical study comparing rat and guinea pig hippocampus.
复制标题
催产素神经元敏感性与催产素受体结合之间的相关性：比较大鼠和豚鼠海马的电生理学和放射自显影研究。
DOI：
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发表时间：
1989
影响因子：
11.1
通讯作者：
J. Dreifuss
中科院分区：
文献类型：
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作者：
M. Raggenbass;E. Tribollet;M. Dubois‐Dauphin;J. Dreifuss
在大鼠和豚鼠大脑的横向海马切片中，我们获得了单一的细胞外记录位于或附近的CA 1领域和CA 1和下托之间的过渡区的层间的神经元。在大鼠中，这些神经元对50-1000 nM的催产素的反应是放电率的可逆增加。催产素诱导的兴奋被抑制的合成结构类似物，作为一个有效的，选择性的外周受体antioxytocic。非锥体神经元也兴奋卡巴胆碱在0.5-10 μ M。该化合物的作用是突触后的，并被毒蕈碱拮抗剂阿托品阻断。相比之下，在豚鼠中，非锥体神经元不受催产素的影响，尽管它们会被卡巴胆碱兴奋。使用放射性碘标记的选择性antioxytocic作为配体进行的光镜放射自显影，显示在海马下托和大鼠海马CA 1区的标记，而在豚鼠海马中没有检测到催产素结合位点。我们的研究结果表明：（i）催产素的海马作用是物种依赖性的，（ii）神经元对催产素的反应与海马中存在的高亲和力结合位点之间存在正相关。
In transverse hippocampal slices from rat and guinea pig brains, we obtained unitary extracellular recordings from nonpyramidal neurones located in or near the stratum pyramidale in the CA1 field and in the transition region between the CA1 and the subiculum. In rats, these neurones responded to oxytocin at 50-1000 nM by a reversible increase in firing rate. The oxytocin-induced excitation was suppressed by a synthetic structural analogue that acts as a potent, selective antioxytocic on peripheral receptors. Nonpyramidal neurones were also excited by carbachol at 0.5-10 microM. The effect of this compound was postsynaptic and was blocked by the muscarinic antagonist atropine. In guinea pigs, by contrast, nonpyramidal neurones were unaffected by oxytocin, although they were excited by carbachol. Light microscopic autoradiography, carried out using a radioiodinated selective antioxytocic as a ligand, revealed labeling in the subiculum and in the CA1 area of the hippocampus of rats, whereas no oxytocin-binding sites were detected in the hippocampus of guinea pigs. Our results indicate (i) that a hippocampal action of oxytocin is species-dependent and (ii) that a positive correlation exists between neuronal responsiveness to oxytocin and the presence in the hippocampus of high-affinity binding sites for this peptide.