Demonstration of the role of prostate-specific antigen in semen liquefaction by two-dimensional electrophoresis.
Demonstration of the role of prostate-specific antigen in semen liquefaction by two-dimensional electrophoresis.
复制标题
通过二维电泳证明前列腺特异性抗原在精液液化中的作用。
DOI：
10.1002/j.1939-4640.1989.tb00134.x
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发表时间：
1989
影响因子：
--
通讯作者：
Sensibar,J
中科院分区：
文献类型：
--
作者：
Lee,C;Keefer,M;Zhao,ZW;Kroes,R;Berg,L;Liu,XX;Sensibar,J
比较了人类精液、前列腺液和精囊液的二维蛋白质谱，以证明液化前后人类精液蛋白质组成的变化。精液标本取自志愿者。前列腺液标本收集直肠按摩患者访问泌尿科诊所。通过针吸法从手术时取出的离体精囊中收集精囊液样本。所有样本均根据ISO ‐ DALT系统制备和处理，用于在二维凝胶中分离蛋白质。电泳后，通过银染色观察凝胶中的蛋白质斑点。前列腺液和精囊液显示出各自的特征性蛋白质谱。人精液蛋白质谱中含有前列腺液和精囊液的特异性蛋白质。精囊液中的一组主要蛋白质（分子量28，000 - 68，000道尔顿），被指定为精囊特异性抗原，在新鲜射精的人精液中观察到，但当精液在室温下静置30分钟时，其二维轮廓消失。当前列腺液或前列腺特异性抗原与精囊液混合并在37 ℃下孵育30分钟时，min时，精囊特异性抗原也从二维图谱中消失。研究结果表明，精囊特异性抗原是精囊液中的一组主要蛋白质，是精液凝固物的结构成分，前列腺特异性抗原是使精囊特异性抗原溶解并液化精液凝固物的酶。
Two‐dimensional protein profiles of human semen, prostatic fluid, and seminal vesicle fluid were compared to demonstrate changes in the protein composition of human semen before and after liquefaction. Semen specimens were obtained from a volunteer. Prostatic fluid specimens were collected by rectal massage from patients visiting a urology clinic. Samples of seminal vesicle fluid were collected by needle aspiration from isolated seminal vesicles, which were removed at surgery. All specimens were prepared and processed according to the ISO‐DALT system for separation of proteins in two‐dimensional gels. Following electrophoresis, protein spots in the gels were visualized by silver staining. Prostatic fluid and seminal vesicle fluid showed their characteristic protein profiles. The protein profile of human semen contained specific proteins of both prostatic fluid and seminal vesicle fluid. One major group of proteins in seminal vesicle fluid (Mw 28,000–68,000 daltons), designated as seminal vesicle‐specific antigen, was observed in freshly ejaculated human semen, but disappeared from the two‐dimensional profile when the ejaculate was allowed to stand at room temperature for 30 min. When prostatic fluid or prostate‐specific antigen was mixed with seminal vesicle fluid and incubated at 37C for 30 min, the seminal vesicle‐specific antigen also disappeared from the two‐dimensional profiles. The findings indicate that seminal vesicle‐specific antigen, a group of predominant proteins in seminal vesicle fluid, is the structural component of seminal coagulum, and that prostate‐specific antigen is the enzyme which digests seminal vesicle‐specific antigen and liquifies semen coagulum.