PROTECTIVE IMMUNITY INDUCED IN SQUIRREL-MONKEYS WITH RECOMBINANT APICAL MEMBRANE ANTIGEN-1 OF PLASMODIUM FRAGILE
PROTECTIVE IMMUNITY INDUCED IN SQUIRREL-MONKEYS WITH RECOMBINANT APICAL MEMBRANE ANTIGEN-1 OF PLASMODIUM FRAGILE
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DOI：
10.4269/ajtmh.1994.51.711
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发表时间：
1994-12-01
影响因子：
3.3
通讯作者：
ANDERS, RF
中科院分区：
文献类型：
--
作者：
COLLINS, WE;PYE, D;ANDERS, RF
采用杆状病毒表达系统制备的易碎型裂殖子尖膜抗原-1与Montanide ISA 720佐剂联合免疫玻利维亚猴。在三次免疫接种后，对猴子进行10,000种脆弱的滋养体寄生虫的攻击。荧光显微镜测定的抗体滴度表明，第二次免疫后反应增强。5只对照动物中有4只在攻毒后18-26天寄生虫计数达到5%。5只接受免疫的猴子中，有4只的最大寄生虫血症水平降低，或寄生虫累积计数延迟，这表明有保护作用。用恶性疟原虫再攻毒后，4只佐剂对照动物中有3只出现了明显的寄生虫病，而5只免疫动物均未感染，提示存在一定程度的异源保护作用。
Saimiri sciureus boliviensis monkeys were immunized with the Plasmodium fragile form of the merozoite apical membrane antigen-1 produced using the baculovirus expression system and combined with Montanide ISA 720 adjuvant. Following three immunizations, monkeys were challenged with 10,000 P. fragile trophozoite parasites. Antibody titers determined by fluorescence microscopy indicated an enhanced response following the second immunization. Four of five control animals had parasite counts > 5% 18-26 days following challenge. Four of five immunized monkeys had reduced levels of maximum parasitemia or delays in accumulated parasite counts, suggestive of protection. Rechallenge of the animals with P. falciparum resulted in three of four adjuvant control animals developing patent parasitemia whereas none of five immunized animals were infected, suggesting some level of heterologous protection.