Concordant and Heterogeneity of Single-Cell Transcriptome in Cardiac Development of Human and Mouse.
Concordant and Heterogeneity of Single-Cell Transcriptome in Cardiac Development of Human and Mouse.
复制标题
人类和小鼠心脏发育中单细胞转录组的一致性和异质性
DOI：
10.3389/fgene.2022.892766
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发表时间：
2022
影响因子：
3.7
通讯作者：
中科院分区：
文献类型：
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作者：
心脏的正常发育对维持其功能至关重要，而心脏的发育过程涉及不同细胞系之间的复杂相互作用。哺乳动物的心脏是如何不同地发育的，目前还没有完全弄清楚。在这项研究中，我们根据人和小鼠胚胎心脏的单细胞转录组数据，鉴定了几种主要类型的心肌细胞，包括心肌细胞(CMS)、成纤维细胞(FBS)、内皮细胞(ECs/FBS)、心外膜细胞(EPs)和免疫细胞(巨噬细胞/单核细胞簇，MACs/Monos)。然后，在两个物种的同一细胞类型中确定物种共享和物种特异的标记基因，并通过构建发育轨迹来选择表达模式一致和不同的基因。通过比较人和小鼠的CMS、FBS和ECs/FBS的发育阶段相似性，发现小鼠在e9.5和e10.5的CMS分别在7W和9W时与人类的CMS最相似。E10.5、e13.5和e14.5的小鼠胎牛在6、7和9周龄时与人类细胞相似，而在10周龄时小鼠的e9.5-ECs/胎牛胎牛血清与人类最相似。这些结果为深入研究心肌细胞类型和能够追踪物种间发育轨迹的关键标记提供了资源，这有利于寻找合适的小鼠模型来检测人类心脏生理和相关疾病。
Normal heart development is vital for maintaining its function, and the development process is involved in complex interactions between different cell lineages. How mammalian hearts develop differently is still not fully understood. In this study, we identified several major types of cardiac cells, including cardiomyocytes (CMs), fibroblasts (FBs), endothelial cells (ECs), ECs/FBs, epicardial cells (EPs), and immune cells (macrophage/monocyte cluster, MACs/MONOs), based on single-cell transcriptome data from embryonic hearts of both human and mouse. Then, species-shared and species-specific marker genes were determined in the same cell type between the two species, and the genes with consistent and different expression patterns were also selected by constructing the developmental trajectories. Through a comparison of the development stage similarity of CMs, FBs, and ECs/FBs between humans and mice, it is revealed that CMs at e9.5 and e10.5 of mice are most similar to those of humans at 7 W and 9 W, respectively. Mouse FBs at e10.5, e13.5, and e14.5 are correspondingly more like the same human cells at 6, 7, and 9 W. Moreover, the e9.5-ECs/FBs of mice are most similar to that of humans at 10W. These results provide a resource for understudying cardiac cell types and the crucial markers able to trace developmental trajectories among the species, which is beneficial for finding suitable mouse models to detect human cardiac physiology and related diseases.
影响因子：
4.8
作者：
Sugden PH;Markou T;Fuller SJ;Tham el L;Molkentin JD;Paterson HF;Clerk A
通讯作者：
Clerk A