Enhanced photodynamic effects using fractionated laser light
Enhanced photodynamic effects using fractionated laser light
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DOI：
10.1016/s1011-1344(97)00124-3
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发表时间：
1998-01-01
影响因子：
5.4
通讯作者：
Haller, U
中科院分区：
文献类型：
--
作者：
Muller, S;Walt, H;Haller, U
肿瘤细胞的光动力根除依赖于光敏剂的存在和对细胞的光传递。本研究调查了分选光（开关模式）对细胞杀伤的影响，这是由一个集落形成试验记录的。光敏剂为m-THPC（乙醇溶，Foscan(R)）和m-THPC- md（水溶性，均来自英国吉尔福德的Scotia制药公司）。对于两种光敏剂，波长为652nm、光持续时间为0.05 s的分选激光比相同功率密度下的连续光照更有效。我们提出，由于在黑暗间隔期间恢复的阶段较短，分步激光的毒性更大，可能在这些条件下导致更多的单线态氧。例如，通过使用Foscan(R)和分馏激光，预计在治疗实体肿瘤方面也会产生类似的效果。在这种情况下，我们期望通过降低光敏剂浓度和/或减少施加的光剂量来改善患者的光动力治疗（PDT）。(C) 1998 Elsevier Science S.A.
Photodynamic eradication of tumour cells depends on the presence of a photosensitizer and light delivery to the cells. The present study investigated the influence of fractionated light (on-off mode) on cell killing as documented by a colony-forming assay. Photosensitizers were m-THPC (ethanol soluble, Foscan(R)) and m-THPC-MD (water soluble, both from Scotia Pharmaceuticals, Guildford, UK). Fractionated laser light at a wavelength of 652 nm with a light duration of 0.05 s was more effective than continuous illumination at the same power density for both photosensitizers. We propose that fractionated laser light is more toxic due to short phases of recovery during the dark intervals, probably resulting in more singlet oxygen under these conditions. By use of Foscan(R), for example, and fractionated laser light, a similar effect is expected for the treatment of solid tumours. In this case we expect improvements in photodynamic therapy (PDT) for patients by lowering the concentrations of photosensitizer and/or by reducing the applied light dose. (C) 1998 Elsevier Science S.A.