REDISTRIBUTION OF INTERMEDIATE FILAMENTS DURING CAPPING OF LYMPHOCYTE SURFACE MOLECULES
REDISTRIBUTION OF INTERMEDIATE FILAMENTS DURING CAPPING OF LYMPHOCYTE SURFACE MOLECULES
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DOI：
10.1038/298284a0
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发表时间：
1982-01-01
期刊：
影响因子：
64.8
通讯作者：
BROUET, JC
中科院分区：
文献类型：
--
作者：
DELLAGI, K;BROUET, JC
Intermediate filaments (IF) constitute a major cytoplasmic filamentous network of higher eukaryotic cells that is distinct from actin and myosin microfilaments or microtubules. Although structurally similar, these filaments are formed by chemically and antigenically different proteins. Vimentin is the major IF polypeptide of mesenchymal cells and cultured non-mesenchymal cell lines1. Recently, we have characterized a monoclonal IgM antibody from a patient with Waldenström's macroglobulinaemia which is directed against vimentin2. Using this monoclonal antibody, we have shown by direct immunofluorescence that intermediate filaments of human B and T lymphocytes consist of vimentin. In cells exposed to colcemid, the intermediate filaments retracted into a juxta-nuclear aggregate (‘coil’) characteristic of vimentin filaments. As most components of the cytoskeleton, especially actin3and myosin4, have been implicated in the capping phenomenon, we investigated the effect of capping of eitherβ2-micro globulin or membrane immunoglobulins on the organization of the intermediate filament network. We report that capping of these surface molecules induced the redistribution of vimentin just beneath the cap. When colcemid-treated cells were allowed to cap, the location of the cap always coincided with the coil, suggesting that the anchorage point of intermediate filaments is situated within the uropod.
