Role of systemic T-cells and histopathological aspects after subcutaneous implantation of various carbon nanotubes in mice
Role of systemic T-cells and histopathological aspects after subcutaneous implantation of various carbon nanotubes in mice
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DOI：
10.1016/j.carbon.2005.08.006
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发表时间：
2006-05-01
期刊：
影响因子：
10.9
通讯作者：
Kuroiwa, N
中科院分区：
文献类型：
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作者：
Koyama, S;Endo, M;Kuroiwa, N
我们通过测量外周血中的CD4（+）和CD8(+) t细胞，以及对皮下植入碳纳米管周围组织长达3个月的组织病理学研究，评估了对四种不同类型碳纳米管（CNTs）的生物学反应。所有小鼠都存活了下来，在我们的实验期间没有观察到体重的大变化。1周后，只有单壁碳纳米管（SWNTs）激活了抗原-抗体反应系统的主要组织相容性复合体（MHC） I类途径(CD4（+)/CD8（+）值升高），导致水肿的出现。2周后，CD4（+）和CD4(+)/CD8（+）显著升高，而CD8（+）没有变化，这表明所有样本的MHC II类被激活。值得注意的是，CNTs的毒理学反应绝对低于石棉。因此，我们设想我们的研究结果（CNTs的毒性相对较低）将在不久的将来促进CNTs的批量生产和广泛应用。(c) 2005 Elsevier Ltd版权所有。
We have evaluated the biological responses to four different types of carbon nanotubes (CNTs), by measuring CD4(+) and CD8(+) T-cells in peripheral blood, and by the histopathological study on tissues surrounding Subcutaneously implanted CNTs for up to 3 months. All mice survived, and no large changes in their weights were observed within our experimental period. After 1 week, only single-walled carbon nanotubes (SWNTs) activated major histocompatibility complex (MHC) class I pathway of antigen-antibody response system (higher CD4(+)/CD8(+) value), resulting in the appearance of an edematous aspect. After 2 weeks, significantly high values in CD4(+) and CD4(+)/CD8(+) without change in CD8(+) signified an activated MHC class II for all samples. It is worth noting that the toxicological response of CNTs was absolutely lower than that of asbestos. As a result, we envisaged that our result (relatively low toxicity of CNTs) will spur the mass-production, as well widespread application of CNTs in the near future. (c) 2005 Elsevier Ltd. All rights reserved.