Surface modification of titanium-based alloys with bioactive molecules using electrochemically fixed nucleic acids
Surface modification of titanium-based alloys with bioactive molecules using electrochemically fixed nucleic acids
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DOI：
10.1002/jbm.b.30579
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发表时间：
2007-01-01
影响因子：
3.4
通讯作者：
Schwenzer, B.
中科院分区：
文献类型：
--
作者：
Michael, J.;Beutner, R.;Schwenzer, B.
提出了一种利用生物活性分子对钛（合金）进行表面改性的新方法，旨在为满足某些医学适应症的特殊要求提供一种新的植入物适应性基础。通过在Ti6 Al 7 Nb阳极上生长氧化物层，通过其末端（区域特异性地）电化学固定核酸单链。它可以表明，它们是可访问的后续杂交与互补链在生理pH值。固定化和杂交的核酸的量进行了测定放射性分析使用P-32标记的核酸。在4V-SCE及以上电位下固定稳定。核酸单链的固定量可达4pmol/cm ~ 2，杂交效率可达1.0。杂交效率被发现依赖于固定的寡核苷酸的表面密度，而杂交率增加时，MgCl 2加入。合成了由与固定链互补的寡核苷酸和具有RGD作为整联蛋白识别位点的六肽GRGDSP组成的缀合物。该偶联物能够与成骨细胞上的整合素结合。结果表明，该缀合物与固定在Ti 6 Al 7 Nb上的锚链结合的程度与未缀合的互补链相当。(c)2006 Wiley Periodicals，Inc.
A new method of surface modification for titanium (alloys) with bioactive molecules was developed with the intention of providing a new basis of implant adaptation for particular requirements of certain medical indications. Nucleic acid single strands are fixed electrochemically via their termini (regiospecifically) by growing an oxide layer on Ti6Al7Nb anodically. It could be shown that they are accessible to subsequent hybridization with complementary strands at physiological pH. Amount of nucleic acids immobilized and hybridized were determined radioanalytically using P-32-labelled nucleic acids. Stable fixation was attained at and above potentials of 4 V-SCE. Up to 4 pmol/cm(2) of nucleic acid single strands could be immobilized and hybridization efficiencies up to 1.0 were reached. Hybridization efficiency was found to depend on surface density of immobilized oligonucleotides, while hybridization rates increased when MgCl2 was added. A conjugate consisting of an oligonucleotide complementary to the immobilized strand and the hexapeptide GRGDSP with RGD as an integrin recognition site was synthesized. This conjugate was able to bind to integrins on osteoblasts. It was shown that this conjugate binds to the anchor strand fixed on Ti6Al7Nb to an extent comparable with the unconjugated complementary strand. (c) 2006 Wiley Periodicals, Inc.