Epstein‐Barr virus‐associated complement‐fixing and nuclear antigens in Burkitt lymphoma biopsies
Epstein‐Barr virus‐associated complement‐fixing and nuclear antigens in Burkitt lymphoma biopsies
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伯基特淋巴瘤活检中的 Epstein-Barr 病毒相关补体固定和核抗原
DOI：
10.1002/ijc.2910130604
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发表时间：
1974
影响因子：
6.4
通讯作者：
B. Johansson
中科院分区：
文献类型：
--
作者：
B. Reedman;G. Klein;J. H. Pope;M. Walters;J. Hilgers;Surjit Singh;B. Johansson
通过补体结合（CF）试验和抗补体免疫荧光（ACIF）试验，检查伯基特淋巴瘤（BL）活检细胞中的EB-巴尔病毒（EBV）相关抗原。在CF测试中，抗补体因子使得难以测试所有可用的活检。然而，来自19名患者的活组织检查被有效地检测，其中12名（包括来自一名患者的两名）含有与具有EBV抗体的人血清电池反应的抗原，但不与缺乏这种抗体的血清反应。技术困难阻碍了活检中EBV相关抗原的进一步表征。对BL应用ACIF检测显示，13例患者中有11例活检组织中存在EBV相关核抗原。吸收研究表明，活检组织的核抗原与先前在淋巴母细胞系中描述的EBV确定的核抗原（EBNA）在抗原性上密切相关。可以得出结论，BL活检的细胞除了先前描述的EBV相关的膜抗原外，还可能含有EBNA。结果提供了进一步的证据，即来自非洲患者的BL细胞类似于含有EBNA的非生产性淋巴母细胞系，因此似乎被EBV转化。
Cells from Burkitt lymphoma (BL) biopsies were examined for Epstein‐Barr virus (EBV)‐associated antigens by complement fixation (CF) tests and by the anti‐complement immunofluorescence (ACIF) test. In CF tests, anticomplementary factors made it difficult to test all the biopsies available. However, biopsies from 19 patients were effectively tested and 12 of these (including two from one patient) contained antigen reacting with a battery of human sera with antibody to EBV but not with sera lacking such antibody. Technical difficulties prevented further characterization of the EBV‐related antigens in the biopsies. Application of the ACIF test to BL revealed the presence of EBV‐related nuclear antigen in biopsies from 11 of 13 patients. Absorption studies indicated that the nuclear antigens of the biopsies were closely related antigenically to the EBV‐determined nuclear antigen (EBNA) previously described in lymphoblastoid cell lines. It is concluded that cells of BL biopsies may contain EBNA in addition to the EBV‐related membrane antigen previously described. The results provide further evidence that BL cells from African patients resemble non‐producer lymphoblastoid cell lines in containing EBNA and therefore appear to be transformed by EBV.