Development of heat-stable recombinant rinderpest vaccine
Development of heat-stable recombinant rinderpest vaccine
复制标题
热稳定重组牛瘟疫苗的研制
DOI：
10.1007/bf01317919
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发表时间：
2005
影响因子：
2.7
通讯作者：
K. Yamanouchi
中科院分区：
文献类型：
--
作者：
K. Tsukiyama;Y. Yoshikawa;H. Kamata;K. Imaoka;K. Asano;S. Funahashi;T. Maruyama;H. Shida;M. Sugimoto;K. Yamanouchi
构建了含有牛瘟病毒（RV）血凝素（H）基因全长cDNA的重组痘苗病毒（RVV）。用两种不同的启动子，即牛痘病毒A型包涵体（ATI）启动子和VV7.5千道尔顿（P7.5）启动子，将H基因插入到痘苗病毒（VV）弱毒疫苗株Lc16m0中。这些RVV在RK13细胞中产生与真实RV-H相同大小的完全糖基化RV-H蛋白。它们在冻干状态下的热稳定性与亲本VV相似。用这些RVV免疫家兔，均可产生RV中和抗体和RV-H抗体。免疫后4周，这些动物用RV静脉内攻击。除了一只用含有ATI启动子的RVV免疫并产生低VN滴度的兔外，没有一只RVV免疫的兔产生RV感染的任何临床体征。这些结果表明，有可能开发一种热稳定的重组疫苗，用于在没有冷藏系统的热带国家根除牛瘟。
Recombinant vaccinia virus (RVV) containing the full-length cDNA of rinderpest virus (RV)-haemagglutinin (H) gene was constructed. The H gene was inserted into the attenuated vaccine strain of vaccinia virus (VV), Lc 16 m0, with two different promoters, namely cowpox virus A-type inclusion body (ATI) promoter or VV 7.5 kilodalton (P7.5) promoter. These RVVs produced the same sized fully glycosylated RV-H protein in RK 13 cells as that of the authentic RV-H. Their heat stability in the lyophylized state was similar to that of the parental VV. All rabbits immunized with these RVVs produced virus neutralizing (VN) antibody to RV as well as anti RV-H antibody. Four weeks after immunization, these animals were challenged with RV intravenously. None of the RVV-immunized rabbits developed any clinical signs of RV infection except one which was immunized with RVV containing the ATI promoter and developed low VN titer. These results indicate the possibility of developing a heat-stable recombinant vaccine for the eradication of rinderpest in tropical countries without cold storage systems.