PHEROMONE RESPONSE ELEMENTS ARE NECESSARY AND SUFFICIENT FOR BASAL AND PHEROMONE-INDUCED TRANSCRIPTION OF THE FUS1 GENE OF SACCHAROMYCES-CEREVISIAE
PHEROMONE RESPONSE ELEMENTS ARE NECESSARY AND SUFFICIENT FOR BASAL AND PHEROMONE-INDUCED TRANSCRIPTION OF THE FUS1 GENE OF SACCHAROMYCES-CEREVISIAE
复制标题
DOI：
10.1128/mcb.11.6.2952
复制
发表时间：
1991-06-01
影响因子：
5.3
通讯作者：
SPRAGUE, GF
中科院分区：
文献类型：
--
作者：
HAGEN, DC;MCCAFFREY, G;SPRAGUE, GF
酿酒酵母的FUS 1基因在α和α-细胞中转录，而不在α/α-二倍体中转录，并且当单倍体细胞暴露于适当的交配信息素时，其转录显著增加。 此外，FUS 1转录绝对依赖于STE 4、STE 5、STE 7、STE 11和STE 12，这些基因被认为编码信息素反应途径的成分。 我们现在已经确定，信息素反应元件（PRE），这发生在FUS 1上游区域内的四个副本，作为FUS 1上游激活序列（UAS）的功能，并负责FUS 1调控的所有已知方面。 特别是，55 bp的删除，其中包括取消所有的转录，和139 bp的片段，其中包括PRE赋予FUS 1样表达的CYC 1-lacZ报告基因。 此外，合成PRE的三个或四个拷贝密切模仿由139-bp片段赋予的活性，并且甚至PRE的单个拷贝赋予了单倍体特异性和信息素诱导的痕量活性。 在FUS 1启动子背景下，在55 bp缺失位点插入的合成PRE的四个拷贝恢复了FUS 1的完整转录。 PRE簇的上游和下游序列对于最大化PRE定向表达是重要的，但其本身不具有UAS活性。 其他具有PRE的酵母基因，例如，STE 2和BAR 1是更适度诱导的，并且具有有助于整体活性的额外UAS元件。 在FUS 1启动子中，PRE显然单独作用以赋予由信息素高度刺激的活性。
The FUS1 gene of Saccharomyces cerevisiae is transcribed in a and alpha-cells, not in a/alpha-diploids, and its transcription increases dramatically when haploid cells are exposed to the appropriate mating pheromone. In addition, FUS1 transcription is absolutely dependent on STE4, STE5, STE7, STE11, and STE12, genes thought to encode components of the pheromone response pathway. We now have determined that the pheromone response element (PRE), which occurs in four copies within the FUS1 upstream region, functions as the FUS1 upstream activation sequence (UAS) and is responsible for all known aspects of FUS1 regulation. In particular, deletion of 55 bp that includes the PREs abolished all transcription, and a 139-bp fragment that includes the PREs conferred FUS1-like expression to a CYC1-lacZ reporter gene. Moreover, three or four copies of a synthetic PRE closely mimicked the activity conferred by the 139-bp fragment, and even a single copy of PRE conferred a trace of activity that was haploid specific and pheromone inducible. In the FUS1 promoter context, four copies of the synthetic PRE inserted at the site of the 55-bp deletion restored full FUS1 transcription. Sequences upstream and downstream from the PRE cluster were important for maximal PRE-directed expression but, by themselves, did not have UAS activity. Other yeast genes with PREs, e.g., STE2 and BAR1, are more modestly inducible and have additional UAS elements contributing to the overall activity. In the FUS1 promoter, the PREs apparently act alone to confer activity that is highly stimulated by pheromone.