Metabolomic profiling of Drosophila using liquid chromatography Fourier transform mass spectrometry
Metabolomic profiling of Drosophila using liquid chromatography Fourier transform mass spectrometry
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DOI：
10.1016/j.febslet.2008.07.029
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发表时间：
2008-08-20
期刊：
影响因子：
3.5
通讯作者：
Watson, D. G.
中科院分区：
文献类型：
--
作者：
Kamleh, M. A.;Hobani, Y.;Watson, D. G.
采用亲水性相互作用色谱（HILIC）与Orbitrap傅立叶变换质谱仪（FT-MS）相结合的方法，对果蝇经典突变玫瑰色（ry）进行了代谢组学分析。该基因编码黄嘌呤氧化酶/脱氢酶。除了通过检测经典报道的黄嘌呤、次黄嘌呤、尿酸盐和尿囊素的相同变化来验证该技术外，在色氨酸、精氨酸、嘧啶和甘油磷脂代谢途径中检测到完全意想不到的变化。因此，这种玫瑰色突变的影响范围比以前发现的要广泛得多。(C) 2008年欧洲生化学会联合会。Elsevier b.v.版权所有。
Hydrophilic interaction chromatography (HILIC) interfaced with an Orbitrap Fourier transform mass spectrometer (FT-MS) was used to carry out metabolomic profiling of the classical Drosophila mutation, rosy (ry). This gene encodes a xanthine oxidase/dehydrogenase. In addition to validating the technology by detecting the same changes in xanthine, hypoxanthine, urate and allantoin that have been reported classically, completely unsuspected changes were detected in each of the tryptophan, arginine, pyrimidine and glycerophospholipid metabolism pathways. The rosy mutation thus ramifies far more widely than previously detected. (C) 2008 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.