Dopamine modulates hemocyte phagocytosis via a D1-like receptor in the rice stem borer, Chilo suppressalis.
Dopamine modulates hemocyte phagocytosis via a D1-like receptor in the rice stem borer, Chilo suppressalis.
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多巴胺通过水稻二化螟中的 D1 样受体调节血细胞吞噬作用
DOI：
10.1038/srep12247
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发表时间：
2015-07-16
影响因子：
4.6
通讯作者：
Ye GY
中科院分区：
文献类型：
--
作者：
Wu SF;Xu G;Stanley D;Huang J;Ye GY
多巴胺（DA）是连接神经和免疫系统的信号片段。DA失调与严重的人类疾病有关，包括成瘾、精神分裂症和帕金森病。然而，DA在免疫系统中的作用仍不完全清楚。在这项研究中，我们发现DA通过从水稻茎螟虫（Chilo suppressalis）中提取的血细胞来调节昆虫的血细胞吞噬。我们研究了昆虫血细胞是否能够从头产生DA。本研究表明，将血细胞暴露于脂多糖（LPS）会诱导da生成酶。外源性DA诱导naïve血细胞细胞外信号调节激酶（ERK）的快速磷酸化。用DOP1受体拮抗剂预孵育可抑制ERK的活化。因此，通过DOP1受体的DA信号可能有助于早期血细胞活化。从血细胞中合成和释放的DA可能以自分泌机制刺激或维持吞噬活性。与这一假设一致，我们发现α-甲基- dl -酪氨酸甲酯盐酸盐抑制DA合成或拮抗剂SCH23390阻断DOP1受体会损害血细胞吞噬。局部DA应用也显著降低了球孢白僵菌侵染后的RSB死亡率。我们推断，一个依赖da的信号系统在血细胞中起调节吞噬功能的作用。
Dopamine (DA) is a signal moiety bridging the nervous and immune systems. DA dysregulation is linked to serious human diseases, including addiction, schizophrenia, and Parkinson’s disease. However, DA actions in the immune system remain incompletely understood. In this study, we found that DA modulates insect hemocyte phagocytosis using hemocytes prepared from the rice stem borer (RSB), Chilo suppressalis. We investigated whether insect hemocytes are capable of de novo DA production. Here we show that exposing hemocytes to lipopolysaccharide (LPS) led to induction of DA-generating enzymes. Exogenous DA induced rapid phosphorylation of extracellular signal-regulated kinase (ERK) in naïve hemocytes. Activation of ERK was inhibited by preincubating with a DOP1 receptor antagonist. Thus, DA signaling via the DOP1 receptor may contribute to early hemocyte activation. DA synthesized and released from hemocytes may act in an autocrine mechanism to stimulate or maintain phagocytic activity. Consistent with this hypothesis, we found that inhibition of DA synthesis with α-methyl-DL-tyrosine methyl ester hydrochloride or blockage of DOP1 receptor with antagonist SCH23390 impaired hemocyte phagocytosis. Topical DA application also significantly decreased RSB mortality following challenge with the insect pathogenic fungus, Beauveria bassiana. We infer that a DA-dependent signaling system operates in hemocytes to mediate phagocytotic functions.