Structure and expression of the human septin gene HCDCREL-1.
Structure and expression of the human septin gene HCDCREL-1.
复制标题
人 septin 基因 HCDCREL-1 的结构和表达。
DOI：
10.1016/s0378-1119(98)00146-2
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发表时间：
1998
期刊：
影响因子：
3.5
通讯作者：
Ware,J
中科院分区：
文献类型：
--
作者：
Yagi,M;Zieger,B;Roth,GJ;Ware,J
Septins是一个高度保守的蛋白质家族，在真菌和果蝇中介导胞质分裂和细胞骨架组织。编码人septin家族成员HCDCREL-1的基因已显示从紧邻血小板糖蛋白（GP）Ib β的基因座转录。HCDCREL-1基因具有非共有多聚腺苷酸化信号，该信号显然未被有效利用，导致还含有血小板GPIb β编码区的通读转录物的表达。作为理解HCDCREL-1的调控和功能的第一步，我们分析了该基因的结构，并表征了其在多种人类细胞中的表达。我们的研究结果表明，该基因在血小板和神经组织中高水平表达，是转录复杂。
Septins are a family of highly conserved filament-forming proteins that have been shown to mediate cytokinesis and cytoskeletal organization in fungi and Drosophila. The gene encoding the human septin family member HCDCREL-1 has been shown to be transcribed from a locus immediately adjacent to that of the platelet glycoprotein (GP) Ib β. The HCDCREL-1 gene possesses a non-consensus polyadenylation signal that apparently is not efficiently utilized, resulting in the expression of a readthrough transcript also containing the platelet GPIb β coding region. As a first step in understanding the regulation and function of HCDCREL-1, we have analyzed the structure of this gene and characterized its expression in a variety of human cells. Our results indicate that the gene is expressed at high levels in platelets and neural tissue, and is transcriptionally complex.